Facile preparation of carbohydrate microarrays by site-specific, covalent immobilization of unmodified carbohydrates on hydrazide-coated glass slides.

[reaction: see text] A new, simple and efficient immobilization method to attach mono-, di-, oligo-, and polysaccharides to hydrazide-coated glass slides was developed. Protein and cell-binding experiments show that the carbohydrate microarrays prepared by this method are applicable for the rapid analysis of protein-carbohydrate interactions and fast detection of pathogens.