OBJECTIVES: We explore the clinical potential of reflectance and fluorescence confocal microscopy to image the morphologic and biochemical changes associated with precancer, in order to aid in the detection and diagnosis of cervical dysplasia. METHODS: Cervical epithelial tissue samples imaged ex vivo or in vivo were obtained from M. D. Anderson Cancer Center and Lyndon B. Johnson Hospital in Houston, Texas. Confocal reflectance microscopy was used to image ex vivo cervical biopsies and in vivo cervical tissue. Confocal fluorescence microscopy was used to image ex vivo cervical tissue slices. RESULTS: We present reflectance and fluorescence confocal images of cervical tissue demonstrating the ability to differentiate between normal and abnormal cervical tissue. CONCLUSIONS: We believe that there is significant clinical potential for confocal microscopy to provide a sensitive and specific method for cervical precancer detection.
OBJECTIVES: We explore the clinical potential of reflectance and fluorescence confocal microscopy to image the morphologic and biochemical changes associated with precancer, in order to aid in the detection and diagnosis of cervical dysplasia. METHODS: Cervical epithelial tissue samples imaged ex vivo or in vivo were obtained from M. D. Anderson Cancer Center and Lyndon B. Johnson Hospital in Houston, Texas. Confocal reflectance microscopy was used to image ex vivo cervical biopsies and in vivo cervical tissue. Confocal fluorescence microscopy was used to image ex vivo cervical tissue slices. RESULTS: We present reflectance and fluorescence confocal images of cervical tissue demonstrating the ability to differentiate between normal and abnormal cervical tissue. CONCLUSIONS: We believe that there is significant clinical potential for confocal microscopy to provide a sensitive and specific method for cervical precancer detection.
Authors: Derek Ho; Tyler K Drake; Karen K Smith-McCune; Teresa M Darragh; Loris Y Hwang; Adam Wax Journal: Int J Cancer Date: 2017-03-15 Impact factor: 7.396
Authors: Dongsuk Shin; Mark C Pierce; Ann M Gillenwater; Michelle D Williams; Rebecca R Richards-Kortum Journal: PLoS One Date: 2010-06-23 Impact factor: 3.240
Authors: Dongkyun Kang; Simon C Schlachter; Robert W Carruth; Minkyu Kim; Tao Wu; Nima Tabatabaei; Paulino Vacas-Jacques; Milen Shishkov; Kevin Woods; Jenny S Sauk; John Leung; Norman S Nishioka; Guillermo J Tearney Journal: Endosc Int Open Date: 2014-07-16
Authors: Fahime Sheikhzadeh; Rabab K Ward; Anita Carraro; Zhao Yang Chen; Dirk van Niekerk; Dianne Miller; Tom Ehlen; Calum E MacAulay; Michele Follen; Pierre M Lane; Martial Guillaud Journal: Biomed Eng Online Date: 2015-10-24 Impact factor: 2.819