BACKGROUND: We have shown previously that the PA-I lectin of Pseudomonas aeruginosa plays a key role in gut-derived sepsis during surgical stress. The aims of this study were to determine if the intestinal tract lumen of a stressed host contained soluble factors that could induce the expression of PA-I. METHODS: Mice were subjected to either 30% surgical hepatectomy or sham-laparotomy, and P. aeruginosa was introduced into the cecum. Twenty-four hours later, feces were recovered, and PA-I and exotoxin A were determined by real-time polymerase chain reaction (PCR). In reiterative experiments, fecal filtrates from both hepatectomy and sham-operated mice were tested for their ability to induce PA-I expression in cultures of P. aeruginosa. Finally, the media from cultured human intestinal epithelial (Caco-2) cells stressed with excess glutamine was tested for its ability to induce the expression of PA-I in cultures of P. aeruginosa. RESULTS: Both PA-I and exotoxin A mRNA were increased in vivo in the intestinal tract of mice subjected to 30% hepatectomy. Soluble fecal filtrates from hepatectomy mice induced PA-I in vitro. Media from epithelial cells exposed to excess glutamine alone induced PA-I expression. CONCLUSIONS: The intestinal environment of a stressed host contains soluble factors capable of inducing lethal virulence traits in human opportunistic pathogen P. aeruginosa.
BACKGROUND: We have shown previously that the PA-I lectin of Pseudomonas aeruginosa plays a key role in gut-derived sepsis during surgical stress. The aims of this study were to determine if the intestinal tract lumen of a stressed host contained soluble factors that could induce the expression of PA-I. METHODS:Mice were subjected to either 30% surgical hepatectomy or sham-laparotomy, and P. aeruginosa was introduced into the cecum. Twenty-four hours later, feces were recovered, and PA-I and exotoxin A were determined by real-time polymerase chain reaction (PCR). In reiterative experiments, fecal filtrates from both hepatectomy and sham-operated mice were tested for their ability to induce PA-I expression in cultures of P. aeruginosa. Finally, the media from cultured human intestinal epithelial (Caco-2) cells stressed with excess glutamine was tested for its ability to induce the expression of PA-I in cultures of P. aeruginosa. RESULTS: Both PA-I and exotoxin A mRNA were increased in vivo in the intestinal tract of mice subjected to 30% hepatectomy. Soluble fecal filtrates from hepatectomy mice induced PA-I in vitro. Media from epithelial cells exposed to excess glutamine alone induced PA-I expression. CONCLUSIONS: The intestinal environment of a stressed host contains soluble factors capable of inducing lethal virulence traits in human opportunistic pathogen P. aeruginosa.
Authors: Katherine A Radek; Peter M Elias; Laurent Taupenot; Sushil K Mahata; Daniel T O'Connor; Richard L Gallo Journal: Cell Host Microbe Date: 2010-04-22 Impact factor: 21.023
Authors: Emily K Cope; Natalia Goldstein-Daruech; Jennifer M Kofonow; Lanette Christensen; Bridget McDermott; Fernando Monroy; James N Palmer; Alexander G Chiu; Mark E Shirtliff; Noam A Cohen; Jeff G Leid Journal: PLoS One Date: 2011-12-05 Impact factor: 3.240
Authors: Olga Zaborina; John C Alverdy; Sanjiv K Hyoju; Alexander Zaborin; Robert Keskey; Anukriti Sharma; Wyatt Arnold; Fons van den Berg; Sangman M Kim; Neil Gottel; Cindy Bethel; Angella Charnot-Katsikas; Peng Jianxin; Carleen Adriaansens; Emily Papazian; Jack A Gilbert Journal: mBio Date: 2019-07-30 Impact factor: 7.867
Authors: Olga Zaborina; Francois Lepine; Gaoping Xiao; Vesta Valuckaite; Yimei Chen; Terry Li; Mae Ciancio; Alex Zaborin; Elaine O Petrof; Elaine Petroff; Jerrold R Turner; Laurence G Rahme; Eugene Chang; John C Alverdy Journal: PLoS Pathog Date: 2007-03 Impact factor: 6.823
Authors: Olga Zaborina; Christopher Holbrook; Yimei Chen; Jason Long; Alexander Zaborin; Irina Morozova; Hoylan Fernandez; Yingmin Wang; Jerrold R Turner; John C Alverdy Journal: PLoS Pathog Date: 2008-02-08 Impact factor: 6.823