Z Dlamini1, K D Bhoola. 1. School of Anatomical Sciences, University of the Witwatersrand, Faculty of Health Sciences, 7 York Road, Parktown, Johannesburg 2193, South Africa. dlaminiz@anatomy.wits.ac.za
Abstract
BACKGROUND: The mitogenic kinin peptides formed by the serine protease, tissue kallikrein (TK1), stimulate the proliferation of tumour cells and, by increasing vascular permeability, enhance metastasis. Oesophageal mucosal epithelial cells are derived from the epithelial cell germ layer, which expresses the kallikrein-kinin cascade. AIM: To determine the cellular distribution of active TK1, prokallikrein, and the kinin B(1) and B(2) receptors in oesophageal carcinoma by immunocytochemistry and in situ hybridisation (ISH). METHODS: Fifty oesophageal specimens (33 biopsies and 17 resections) and 10 control specimens adjacent to tumour or normal oesophageal biopsies were studied. Specific antibodies were used to determine the cellular localisation of TK1, prokallikrein, and the kinin B(1) and B(2) receptors in normal and oesophageal specimens by standard immunohistochemical techniques. The intensity of immunolabelling was quantified by image analysis. Antisense probes for TK1 and the kinin B(1) and B(2) receptors were also used to localise mRNA. RESULTS: TK1 (active and prokallikrein) was expressed in the mucosa of normal and tumour oesophageal epithelium. In general, expression was highest in activated mast cells, followed by giant tumour cells. Immunolabelling results were confirmed by ISH experiments. CONCLUSIONS: This is the first demonstration that TK1 and kinin B(1) and B(2) receptors are expressed in oesophageal carcinoma. Because TK1 released from tumour cells enzymatically generates mitogenic kinins from its endogenous substrate, kininogen, it is possible that third generation kinin receptor antagonists, which have been shown to be cytotoxic to cancer cells, may be useful therapeutic agents in this disease.
BACKGROUND: The mitogenic kinin peptides formed by the serine protease, tissue kallikrein (TK1), stimulate the proliferation of tumour cells and, by increasing vascular permeability, enhance metastasis. Oesophageal mucosal epithelial cells are derived from the epithelial cell germ layer, which expresses the kallikrein-kinin cascade. AIM: To determine the cellular distribution of active TK1, prokallikrein, and the kinin B(1) and B(2) receptors in oesophageal carcinoma by immunocytochemistry and in situ hybridisation (ISH). METHODS: Fifty oesophageal specimens (33 biopsies and 17 resections) and 10 control specimens adjacent to tumour or normal oesophageal biopsies were studied. Specific antibodies were used to determine the cellular localisation of TK1, prokallikrein, and the kinin B(1) and B(2) receptors in normal and oesophageal specimens by standard immunohistochemical techniques. The intensity of immunolabelling was quantified by image analysis. Antisense probes for TK1 and the kinin B(1) and B(2) receptors were also used to localise mRNA. RESULTS:TK1 (active and prokallikrein) was expressed in the mucosa of normal and tumour oesophageal epithelium. In general, expression was highest in activated mast cells, followed by giant tumour cells. Immunolabelling results were confirmed by ISH experiments. CONCLUSIONS: This is the first demonstration that TK1 and kinin B(1) and B(2) receptors are expressed in oesophageal carcinoma. Because TK1 released from tumour cells enzymatically generates mitogenic kinins from its endogenous substrate, kininogen, it is possible that third generation kinin receptor antagonists, which have been shown to be cytotoxic to cancer cells, may be useful therapeutic agents in this disease.
Authors: Carl Kapadia; Albert Chang; Georgia Sotiropoulou; George M Yousef; Linda Grass; Antoninus Soosaipillai; Xuekun Xing; David H C Howarth; Eleftherios P Diamandis Journal: Clin Chem Date: 2003-01 Impact factor: 8.327
Authors: Spyridon Christodoulou; Dimitra K Alexopoulou; Christos K Kontos; Andreas Scorilas; Iordanis N Papadopoulos Journal: Tumour Biol Date: 2014-01-16
Authors: May Hamza; Xiao-Min Wang; Albert Adam; Jaime S Brahim; Janet S Rowan; Gilberto N Carmona; Raymond A Dionne Journal: Mol Pain Date: 2010-02-13 Impact factor: 3.395
Authors: P Dominek; P Campagnolo; M H-Zadeh; N Kränkel; M Chilosi; J A Sharman; A Caporali; G Mangialardi; G Spinetti; C Emanueli; M Pignatelli; P Madeddu Journal: Br J Cancer Date: 2010-09-21 Impact factor: 7.640
Authors: Inga I Kramarenko; Thomas A Morinelli; Marlene A Bunni; John R Raymond; Maria N Garnovskaya Journal: Cancer Manag Res Date: 2012-07-26 Impact factor: 3.989