| Literature DB >> 16126335 |
Kuei-Tien Chen1, Jen-Der Lin, Miaw-Jene Liou, Hsiao-Fen Weng, C Allen Chang, Err-Cheng Chan.
Abstract
Platelet-derived growth factor receptor (PDGFR) can bind to its ligand and consequently possess a kinase activity, and which is associated with the carcinogenesis of different cell types, including astrocytomas, oligodendrogliomas, and glioblastoma. In a cDNA microarray analysis, we observe the over-expressed mRNA of both PDGF-A and PDGF-alpha receptor in thyroid carcinoma cells. And the elevated protein expressions of PDGF-A and PDGF-alpha receptor in thyroid carcinoma cells were also confirmed by a Western blot analysis. The phosphorylation of PDGF-alpha receptor evaluated by an antibody against Tyr 720-phosphate was found in thyroid carcinoma cells. The tyrosine kinase activity of PDGF-alpha receptor was inhibited by tyrphostin AG1295 and showed a dose-dependent inhibition for the proliferation of thyroid carcinoma cells. These findings imply that autocrine activation of PDGF-alpha receptor plays a crucial role in the carcinogenesis of thyroid cells.Entities:
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Year: 2005 PMID： 16126335 DOI： 10.1016/j.canlet.2005.01.039
Source DB: PubMed Journal: Cancer Lett ISSN： 0304-3835 Impact factor: 8.679