UNLABELLED: Cyclooxygenase-2 (COX-2) expression has been found to associate with poor prognosis in several types of carcinomas. HuR is an mRNA stability protein and it regulates the expression of COX-2. OBJECTIVES AND METHODS: We analyzed the expression of COX-2 and HuR in 64 mucinous ovarian carcinoma specimens by immunohistochemistry. RESULTS: In mucinous tumors, high COX-2 protein expression was found in epithelial cancer cells in 39% (22/56) and in stromal cells in 24% (13/55) of the specimens. The expression of COX-2 in cancer cells correlated with high grade (P = 0.0285), but stromal COX-2 expression had no correlation with any clinical parameter tested. Cytoplasmic HuR protein expression was observed in cancer cells in 47% (27/57) and in stromal cells in 7% (4/56) of the mucinous tumors, and it correlated with COX-2 expression in the cancer cells (P = 0.0162) but not in the stroma. CONCLUSION: Our results support the hypothesis that cytoplasmic HuR is connected to COX-2 expression in ovarian carcinoma, but that its role is restricted to the transformed epithelial cancer cells.
UNLABELLED: Cyclooxygenase-2 (COX-2) expression has been found to associate with poor prognosis in several types of carcinomas. HuR is an mRNA stability protein and it regulates the expression of COX-2. OBJECTIVES AND METHODS: We analyzed the expression of COX-2 and HuR in 64 mucinous ovarian carcinoma specimens by immunohistochemistry. RESULTS: In mucinous tumors, high COX-2 protein expression was found in epithelial cancer cells in 39% (22/56) and in stromal cells in 24% (13/55) of the specimens. The expression of COX-2 in cancer cells correlated with high grade (P = 0.0285), but stromal COX-2 expression had no correlation with any clinical parameter tested. Cytoplasmic HuR protein expression was observed in cancer cells in 47% (27/57) and in stromal cells in 7% (4/56) of the mucinous tumors, and it correlated with COX-2 expression in the cancer cells (P = 0.0162) but not in the stroma. CONCLUSION: Our results support the hypothesis that cytoplasmic HuR is connected to COX-2 expression in ovarian carcinoma, but that its role is restricted to the transformed epithelial cancer cells.
Authors: S M Sureban; S Ramalingam; G Natarajan; R May; D Subramaniam; K S Bishnupuri; A R Morrison; B K Dieckgraefe; D J Brackett; R G Postier; C W Houchen; S Anant Journal: Oncogene Date: 2008-04-21 Impact factor: 9.867
Authors: T Kurosu; N Ohga; Y Hida; N Maishi; K Akiyama; W Kakuguchi; T Kuroshima; M Kondo; T Akino; Y Totsuka; M Shindoh; F Higashino; K Hida Journal: Br J Cancer Date: 2011-02-01 Impact factor: 7.640
Authors: Fernando F Blanco; Ranjan Preet; Andrea Aguado; Vikalp Vishwakarma; Laura E Stevens; Alok Vyas; Subhash Padhye; Liang Xu; Scott J Weir; Shrikant Anant; Nicole Meisner-Kober; Jonathan R Brody; Dan A Dixon Journal: Oncotarget Date: 2016-11-08