INTRODUCTION: In this paper the activity of cytoplasmatic antioxidant enzymes after multiple freeze-thaw cycles of samples of cytoplasm was assayed in order to characterise the limits that would be necessary to impose on experimental design. METHODS: The activities of antioxidant enzymes in samples of rat liver cytoplasm were evaluated after 1, 2, 3, 4, 5, 10 and 15 freeze-thaw cycles. The activity of antioxidant enzymes was determined by standard methods developed for this purpose. RESULTS: The most resistant against freeze-thaw stress was catalase; even 15 freeze-thaw cycles did not change its activity. The remaining enzymes tested revealed a significant loss in their activity after 4-5 freeze-thaw cycles. The most sensitive was glutathione S-tranferase; its activity was significantly decreased after 3 thaw cycles. DISCUSSION: The data presented may be helpful in designing experiments in which assay of antioxidant enzymes is a part.
INTRODUCTION: In this paper the activity of cytoplasmatic antioxidant enzymes after multiple freeze-thaw cycles of samples of cytoplasm was assayed in order to characterise the limits that would be necessary to impose on experimental design. METHODS: The activities of antioxidant enzymes in samples of rat liver cytoplasm were evaluated after 1, 2, 3, 4, 5, 10 and 15 freeze-thaw cycles. The activity of antioxidant enzymes was determined by standard methods developed for this purpose. RESULTS: The most resistant against freeze-thaw stress was catalase; even 15 freeze-thaw cycles did not change its activity. The remaining enzymes tested revealed a significant loss in their activity after 4-5 freeze-thaw cycles. The most sensitive was glutathione S-tranferase; its activity was significantly decreased after 3 thaw cycles. DISCUSSION: The data presented may be helpful in designing experiments in which assay of antioxidant enzymes is a part.
Authors: Edith Lubos; Neil J Kelly; Scott R Oldebeken; Jane A Leopold; Ying-Yi Zhang; Joseph Loscalzo; Diane E Handy Journal: J Biol Chem Date: 2011-08-18 Impact factor: 5.157
Authors: Renate Schnabel; Edith Lubos; Claudia M Messow; Christoph R Sinning; Tanja Zeller; Philipp S Wild; Dirk Peetz; Diane E Handy; Thomas Munzel; Joseph Loscalzo; Karl J Lackner; Stefan Blankenberg Journal: Am Heart J Date: 2008-12 Impact factor: 4.749
Authors: Jie Li; Donglan Zhang; Pearl A Jefferson; Kathleen M Ward; Iraimoudi S Ayene Journal: J Pharmacol Toxicol Methods Date: 2013-10-20 Impact factor: 1.950