BACKGROUND: Interleukin (IL)-19, a member of the IL-10 family, signals through the IL-20R1/IL-20R2 heterodimer, which is shown to be involved in abnormal keratinocyte differentiation and proliferation. Little is known about its in vitro biological functions or its role in psoriasis. OBJECTIVES: To investigate the role of IL-19 in the psoriatic process. METHODS: The expression of keratinocyte growth factor (KGF) transcripts was measured by polymerase chain reaction in CD8+ T cells treated with IL-19. Next, we developed monoclonal and polyclonal antibodies to measure the levels of IL-19 in the sera of patients with psoriasis and healthy volunteers using an enzyme-linked immunosorbent assay. In addition, we performed immunohistochemical staining on psoriatic skin and normal controls. RESULTS: We found that IL-19 upregulated KGF transcripts on CD8+ T cells. Patients with psoriasis had a lower level of IL-19 in serum than healthy volunteers. The difference between these two groups was statistically significant (P < 0.05). IL-19 expression was seen in basal and suprabasal keratinocytes in a continuous pattern, and was increased in psoriatic epidermis. CONCLUSIONS: These results suggest that IL-19 plays a role in the complex pathological cytokine network in psoriasis.
BACKGROUND:Interleukin (IL)-19, a member of the IL-10 family, signals through the IL-20R1/IL-20R2 heterodimer, which is shown to be involved in abnormal keratinocyte differentiation and proliferation. Little is known about its in vitro biological functions or its role in psoriasis. OBJECTIVES: To investigate the role of IL-19 in the psoriatic process. METHODS: The expression of keratinocyte growth factor (KGF) transcripts was measured by polymerase chain reaction in CD8+ T cells treated with IL-19. Next, we developed monoclonal and polyclonal antibodies to measure the levels of IL-19 in the sera of patients with psoriasis and healthy volunteers using an enzyme-linked immunosorbent assay. In addition, we performed immunohistochemical staining on psoriatic skin and normal controls. RESULTS: We found that IL-19 upregulated KGF transcripts on CD8+ T cells. Patients with psoriasis had a lower level of IL-19 in serum than healthy volunteers. The difference between these two groups was statistically significant (P < 0.05). IL-19 expression was seen in basal and suprabasal keratinocytes in a continuous pattern, and was increased in psoriatic epidermis. CONCLUSIONS: These results suggest that IL-19 plays a role in the complex pathological cytokine network in psoriasis.
Authors: Stephen Ellison; Khatuna Gabunia; Sheri E Kelemen; Ross N England; Rosario Scalia; James M Richards; A Wayne Orr; Wayne Orr; James G Traylor; Thomas Rogers; William Cornwell; Lisa M Berglund; Isabel Goncalves; Maria F Gomez; Michael V Autieri Journal: Arterioscler Thromb Vasc Biol Date: 2013-08-15 Impact factor: 8.311
Authors: R Scrivo; P Conigliaro; V Riccieri; M Di Franco; C Alessandri; A Spadaro; R Perricone; G Valesini Journal: Clin Exp Immunol Date: 2015-02 Impact factor: 4.330