Measurements of the anisotropy of ultrasonic velocity in freshly excised and formalin-fixed myocardial tissue.

The objective of this study was to quantify the anisotropy of ultrasonic velocity in freshly excised myocardial tissue and to examine the effects of formalin-fixation. Through-transmission radio-frequency-based measurements were performed on ovine and bovine myocardial specimens from 24 different hearts. A total of 81 specimens were obtained from specific locations within each heart to investigate the possibility of regional differences in anisotropy of velocity in the left ventricular wall and septum. No regional differences were observed for either lamb or cow myocardial specimens. In addition, no specific species-dependent differences were observed between ovine and bovine myocardium. Average values of velocity at room temperature for perpendicular and parallel insonification were 1556.9 +/- 0.6 and 1565.2 +/- 0.7 m/s (mean +/- standard error), respectively, for bovine myocardium (N=45) and 1556.3 +/- 0.6 and 1564.7 +/- 0.7 m/s for ovine myocardium (N=36). Immediately after measurements of freshly excised myocardium, ovine specimens were fixed in formalin for at least one month and then measurements were repeated. Formalin-fixation appears to increase the overall velocity at all angles of insonification and to increase the magnitude of anisotropy of velocity.