Literature DB >> 16118451

Methods for measuring TGF-b using antibodies, cells, and mice.

Vladimir Jurukovski1, Branka Dabovic, Vesna Todorovic, Yan Chen, Daniel B Rifkin.   

Abstract

The transforming growth factor (TGF)-betas are essential in pre- and postnatal development, differentiation and morphogenesis of higher organisms. Quantitation of the levels of TGF-beta synthesis, secretion, and activation are crucial for grasping the mechanisms that control these events and ultimately control TGF-beta action. Rather than presenting a single method, we describe several methods for measuring active TGF-beta in different experimental situations. This is possible as a result of advances in transgenic mice technology that allow in vivo TGF-beta measurements in addition to the more established in vitro approaches.

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Year:  2005        PMID： 16118451     DOI： 10.1385/1-59259-940-0:161

Source DB:  PubMed          Journal:  Methods Mol Med        ISSN： 1543-1894


  11 in total

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