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Literature DB >> 16118422

Isolation of yeast mitochondria.

Chris Meisinger¹, Nikolaus Pfanner, Kaye N Truscott.

Abstract

Often preparations of isolated organelles contain other, unwanted, cellular components. For biochemical experiments to determine the localization of newly identified proteins, or to determine the whole set of proteins (or the proteome) from a desired organelle, these unwanted components often confuse the resulting data. For these types of studies, it is crucial to have highly pure fractions of the desired organelle. Here we describe a protocol for purification of mitochondria from Saccharomyces cerevisiae cells devoid of contamination from other cellular compartments.

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Year: 2006 PMID： 16118422 DOI： 10.1385/1-59259-958-3:033

Source DB: PubMed Journal: Methods Mol Biol ISSN： 1064-3745

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Cited

127 in total

1. Cryo-slicing Blue Native-Mass Spectrometry (csBN-MS), a Novel Technology for High Resolution Complexome Profiling.

Authors: Catrin S Müller; Wolfgang Bildl; Alexander Haupt; Lars Ellenrieder; Thomas Becker; Carola Hunte; Bernd Fakler; Uwe Schulte
Journal: Mol Cell Proteomics Date: 2015-11-23 Impact factor: 5.911

2. Regulatory role of the respiratory supercomplex factors in Saccharomyces cerevisiae.

Authors: Camilla Rydström Lundin; Christoph von Ballmoos; Martin Ott; Pia Ädelroth; Peter Brzezinski
Journal: Proc Natl Acad Sci U S A Date: 2016-07-18 Impact factor: 11.205

3. Precursor oxidation by Mia40 and Erv1 promotes vectorial transport of proteins into the mitochondrial intermembrane space.

Authors: Judith M Müller; Dusanka Milenkovic; Bernard Guiard; Nikolaus Pfanner; Agnieszka Chacinska
Journal: Mol Biol Cell Date: 2007-10-31 Impact factor: 4.138

4. Shy1 couples Cox1 translational regulation to cytochrome c oxidase assembly.

Authors: David U Mick; Karina Wagner; Martin van der Laan; Ann E Frazier; Inge Perschil; Magdalena Pawlas; Helmut E Meyer; Bettina Warscheid; Peter Rehling
Journal: EMBO J Date: 2007-09-20 Impact factor: 11.598

5. The morphology proteins Mdm12/Mmm1 function in the major beta-barrel assembly pathway of mitochondria.

Authors: Chris Meisinger; Sylvia Pfannschmidt; Michael Rissler; Dusanka Milenkovic; Thomas Becker; Diana Stojanovski; Matthew J Youngman; Robert E Jensen; Agnieszka Chacinska; Bernard Guiard; Nikolaus Pfanner; Nils Wiedemann
Journal: EMBO J Date: 2007-04-05 Impact factor: 11.598

Isolation of yeast mitochondria.

1. Cryo-slicing Blue Native-Mass Spectrometry (csBN-MS), a Novel Technology for High Resolution Complexome Profiling.

2. Regulatory role of the respiratory supercomplex factors in Saccharomyces cerevisiae.

3. Precursor oxidation by Mia40 and Erv1 promotes vectorial transport of proteins into the mitochondrial intermembrane space.

4. Shy1 couples Cox1 translational regulation to cytochrome c oxidase assembly.

5. The morphology proteins Mdm12/Mmm1 function in the major beta-barrel assembly pathway of mitochondria.

6. Identification of the signal directing Tim9 and Tim10 into the intermembrane space of mitochondria.

7. Zinc can play chaperone-like and inhibitor roles during import of mitochondrial small Tim proteins.

8. The heme a synthase Cox15 associates with cytochrome c oxidase assembly intermediates during Cox1 maturation.

9. Overlapping Role of Respiratory Supercomplex Factor Rcf2 and Its N-terminal Homolog Rcf3 in Saccharomyces cerevisiae.

Review 10. Intracellular trafficking of the pyridoxal cofactor. Implications for health and metabolic disease.