Literature DB >> 1611671

Rapid and simple isolation of DNA from agarose gels.

M Grey1, M Brendel.   

Abstract

We present a simple method for the isolation of DNA from agarose gels that is economic, fast, and independent of electrical equipment. DNA fragments of up to 6 kb can be easily extracted within 5 min using a disposable plastic syringe and filter paper. Total extraction of DNA fragments between 10 and 20 kb in size is achieved by concentrating the DNA flushed from the gel in a DNA-binding column.

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Year:  1992        PMID： 1611671     DOI： 10.1007/bf00351746

Source DB:  PubMed          Journal:  Curr Genet        ISSN： 0172-8083            Impact factor:   3.886


  5 in total

1.  A freeze-squeeze method for recovering long DNA from agarose gels.

Authors:  R W Thuring; J P Sanders; P Borst
Journal:  Anal Biochem       Date:  1975-05-26       Impact factor: 3.365

2.  Gel electrophoresis of restriction fragments.

Authors:  E Southern
Journal:  Methods Enzymol       Date:  1979       Impact factor: 1.600

3.  Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.

Authors:  M W McDonell; M N Simon; F W Studier
Journal:  J Mol Biol       Date:  1977-02-15       Impact factor: 5.469

4.  A reliable method for the recovery of DNA fragments from agarose and acrylamide gels.

Authors:  G Dretzen; M Bellard; P Sassone-Corsi; P Chambon
Journal:  Anal Biochem       Date:  1981-04       Impact factor: 3.365

5.  Recovery of DNA from gels.

Authors:  H O Smith
Journal:  Methods Enzymol       Date:  1980       Impact factor: 1.600
  5 in total
  3 in total

1.  A quick and effective in-house method of DNA purification from agarose gel, suitable for sequencing.

Authors:  Obrador-Sánchez José Abraham; Tzec-Sima Miguel; Higuera-Ciapara Inocencio; Canto-Canché Blondy
Journal:  3 Biotech       Date:  2017-06-29       Impact factor: 2.406

2.  Purification of DNA fragments from lyophilized agarose gels.

Authors:  M Benore-Parsons; L Anderson
Journal:  Nucleic Acids Res       Date:  1995-12-11       Impact factor: 16.971

3.  Gene SNQ2 of Saccharomyces cerevisiae, which confers resistance to 4-nitroquinoline-N-oxide and other chemicals, encodes a 169 kDa protein homologous to ATP-dependent permeases.

Authors:  J Servos; E Haase; M Brendel
Journal:  Mol Gen Genet       Date:  1993-01
  3 in total

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