AIM: To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic transdifferentiation of osteoblasts. METHODS: MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed. RESULTS: Daidzin, genistin, and glycitin 1*10(-8), 5*10(-7), 1*10(-6), 5*10(-6), and 1*10(-5) mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5*10(-7) mol/L and genistin 1*10(-6) mol/L promoted the osteogenesis of MSC. Genistin 1*10(-8), 5*10(-7), 1*10(-6), 5*10(-6), and 1*10(-5) mol/L and glycitin 1*10(-8), 1*10(-6), and 1*10(-5) mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1*10(-8), 5*10(-7), 1*10(-6), 5*10(-6), and 1*10(-5) mol/L all inhibited the adipocytic transdifferentiation of osteoblasts. CONCLUSIONS: Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferen-tiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis.
AIM: To examine the effect of daidzin, genistin, and glycitin on the osteogenic and adipogenic differentiation of bone marrow stromal cells (MSC) and the adipogenic transdifferentiation of osteoblasts. METHODS:MTT test, alkaline phosphatase (ALP) activity measurement, Oil Red O stain and measurement were employed. RESULTS:Daidzin, genistin, and glycitin 1*10(-8), 5*10(-7), 1*10(-6), 5*10(-6), and 1*10(-5) mol/L all promoted the proliferation of primary mouse bone MSC and osteoblasts. Daidzin 5*10(-7) mol/L and genistin 1*10(-6) mol/L promoted the osteogenesis of MSC. Genistin 1*10(-8), 5*10(-7), 1*10(-6), 5*10(-6), and 1*10(-5) mol/L and glycitin 1*10(-8), 1*10(-6), and 1*10(-5) mol/L inhibited the adipogenesis of MSC. Daidzin, genistin, and glycitin 1*10(-8), 5*10(-7), 1*10(-6), 5*10(-6), and 1*10(-5) mol/L all inhibited the adipocytic transdifferentiation of osteoblasts. CONCLUSIONS:Daidzin, genistin, and glycitin may modulate differentiation of MSC to cause a lineage shift toward the osteoblast and away from the adipocytes, and could inhibit adipocytic transdifferen-tiation of osteoblasts. They could also be helpful in preventing the development of osteonecrosis.
Authors: Hui H Zhang; Jingxiang Huang; Katrin Düvel; Bernard Boback; Shulin Wu; Rachel M Squillace; Chin-Lee Wu; Brendan D Manning Journal: PLoS One Date: 2009-07-10 Impact factor: 3.240