| Literature DB >> 16112207 |
I A Mechtcheriakova1, M A Eldarov, L Nicholson, M Shanks, K G Skryabin, G P Lomonossoff.
Abstract
The expression and assembly of the hepatitis B virus (HBV) nucleocapsid protein (HBcAg) were investigated in plants using viral vectors. Constructs based on either Potato virus X (PVX) or Cowpea mosaic virus (CPMV) containing the sequence of HBcAg were able to infect the appropriate host plants and remained genetically stable during infection. Analysis of HBcAg expression revealed that the protein can self-assemble into core-like particles and that the assembled material could be partially purified by differential centrifugation. Thus, the use of viral vectors can be considered a practical method for rapid production of assembled HBcAg particles in plants. This approach provides a means whereby a variety of chimaeric particles can be assessed quickly and cheaply for various diagnostic and vaccine applications.Entities:
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Year: 2005 PMID： 16112207 DOI： 10.1016/j.jviromet.2005.06.020
Source DB: PubMed Journal: J Virol Methods ISSN： 0166-0934 Impact factor: 2.014