| Literature DB >> 16108566 |
Jon S Beadell1, Robert C Fleischer.
Abstract
We describe a reliable and relatively inexpensive method for detecting and differentiating between the commonly studied avian blood parasite genera Haemoproteus, Plasmodium, and Leucocytozoon. The assay takes advantage of a Haemoproteus-specific restriction site identified by sequencing full mitochondrial genomes from two Haemoproteus and three Plasmodium lineages and an adjacent, genus-specific restriction site identified in Leucocytozoon spp. The assay was sensitive to simulated parasitemias of approximately 8 x 10(-6) per erythrocyte and was 100% accurate in differentiating between parasite genera isolated from a broad geographical and taxonomic sampling of infected hosts.Entities:
Mesh:
Substances:
Year: 2005 PMID: 16108566 DOI: 10.1645/GE-3412RN
Source DB: PubMed Journal: J Parasitol ISSN: 0022-3395 Impact factor: 1.276