BACKGROUND AND PURPOSE: Endothelial nitric oxide exerts a variety of protective effects on endothelial cells and blood vessels, and therefore the nitric oxide synthase 3 gene (NOS3) is a logical candidate gene for stroke susceptibility. METHODS: We used the population-based Stroke Prevention in Young Women case-control study to assess the association of five NOS3 polymorphisms in 110 cases (46% black) with ischemic stroke and 206 controls (38% black), 15 to 44 years of age. Polymorphisms included 3 single nucleotide polymorphisms (SNPs) in the promoter region (-1468 T>A, -922 G>A, -786 T>C), 1 SNP in exon 7 (G894T), and 1 insertion/deletion polymorphism within intron 4. RESULTS: Significant associations with both the -922 G>A and -786 T>C SNPs with ischemic stroke were observed in the black, but not the white, population. This association was attributable to an increased prevalence of the -922 A allele (OR=3.0, 95% CI=1.3 to 6.8; P=0.005) and the -786 T allele (OR=2.9, 95% CI=1.3 to 6.4; P=0.005) in cases versus controls. These 2 SNPs were in strong linkage disequilibrium (D'=1.0), making it impossible to determine, within the confines of this genetic study, whether 1 or both of these polymorphisms are functionally related to NOS3 expression. Two sets of haplotypes were also identified, 1 of which may confer an increased susceptibility to stroke in blacks, whereas the other appears to be protective. CONCLUSIONS: Promoter variants in NOS3 may be associated with ischemic stroke susceptibility among young black women.
BACKGROUND AND PURPOSE: Endothelial nitric oxide exerts a variety of protective effects on endothelial cells and blood vessels, and therefore the nitric oxide synthase 3 gene (NOS3) is a logical candidate gene for stroke susceptibility. METHODS: We used the population-based Stroke Prevention in Young Women case-control study to assess the association of five NOS3 polymorphisms in 110 cases (46% black) with ischemic stroke and 206 controls (38% black), 15 to 44 years of age. Polymorphisms included 3 single nucleotide polymorphisms (SNPs) in the promoter region (-1468 T>A, -922 G>A, -786 T>C), 1 SNP in exon 7 (G894T), and 1 insertion/deletion polymorphism within intron 4. RESULTS: Significant associations with both the -922 G>A and -786 T>C SNPs with ischemic stroke were observed in the black, but not the white, population. This association was attributable to an increased prevalence of the -922 A allele (OR=3.0, 95% CI=1.3 to 6.8; P=0.005) and the -786 T allele (OR=2.9, 95% CI=1.3 to 6.4; P=0.005) in cases versus controls. These 2 SNPs were in strong linkage disequilibrium (D'=1.0), making it impossible to determine, within the confines of this genetic study, whether 1 or both of these polymorphisms are functionally related to NOS3 expression. Two sets of haplotypes were also identified, 1 of which may confer an increased susceptibility to stroke in blacks, whereas the other appears to be protective. CONCLUSIONS: Promoter variants in NOS3 may be associated with ischemic stroke susceptibility among young black women.
Authors: J Alvarez; J Matias-Guiu; J Sumalla; M Molins; R Insa; J M Moltó; R Martín; A Codina; J M Martinez-Vazquez Journal: Acta Neurol Scand Date: 1989-07 Impact factor: 3.209
Authors: Leah R MacClellan; Timothy D Howard; John W Cole; O Colin Stine; Wayne H Giles; Jeffery R O'Connell; Marcella A Wozniak; Barney J Stern; Braxton D Mitchell; Steven J Kittner Journal: Stroke Date: 2009-08-06 Impact factor: 7.914
Authors: Young Seok Park; Kyung Tae Min; Tae-Gon Kim; Yun Ho Lee; Hee Jin Cheong; In Sun Yeom; Joong-Uhn Choi; Dong-Seok Kim; Nam Keun Kim Journal: Childs Nerv Syst Date: 2011-06-21 Impact factor: 1.475
Authors: Dara G Torgerson; Philip L Ballard; Roberta L Keller; Sam S Oh; Scott Huntsman; Donglei Hu; Celeste Eng; Esteban G Burchard; Roberta A Ballard Journal: Am J Physiol Lung Cell Mol Physiol Date: 2018-08-16 Impact factor: 5.464
Authors: Danhua Du; Peng Gao; Linsen Hu; Yumei Yang; Feng Wang; Lin Ye; Xuan Zhang; Ming Chang; Jiexu Zhao; Jiang Wu; Ian L Megson; Jun Wei Journal: Int J Gen Med Date: 2008-11-30
Authors: Nerissa U Ko; Pam Rajendran; Helen Kim; Martin Rutkowski; Ludmila Pawlikowska; Pui-Yan Kwok; Randall T Higashida; Michael T Lawton; Wade S Smith; Jonathan G Zaroff; William L Young Journal: Stroke Date: 2008-02-28 Impact factor: 7.914