Literature DB >> 161

Rapid kinetic measurement of lactate in plasma with a centrifugal analyzer.

M A Pesce, S H Bodourian, J F Nicholson.   

Abstract

In this method, blood is collected in ammonium heparinized microhematocrit tubes and lactate is directly determined in the plasma, separated within 15 min from the erythrocytes. Lactate is assayed by mixing 10 mul of sample with NAD+ and lactate dehydrogenase in tris(hydroxymethyl)aminomethane hydrazine buffer. The rate of increase in absorbance of the NADH formed, measured at 340 nm, is proportional to lactate concentration. The assay is complete in 4 min and absorbance is linearly related to concentration from 0.625 to 15 mmol/liter. Analytical recoveries of lactate added to plasma averaged 104% (range, 91-116%). Results compared well for plasma samples analyzed by this method with the CentrifiChem and the Du Pont aca.

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Year:  1975        PMID： 161

Source DB:  PubMed          Journal:  Clin Chem        ISSN： 0009-9147            Impact factor:   8.327


  7 in total

1.  Independent AMP and NAD signaling regulates C2C12 differentiation and metabolic adaptation.

Authors:  Chia George Hsu; Thomas J Burkholder
Journal:  J Physiol Biochem       Date:  2016-07-08       Impact factor: 4.158

2.  A rapid blood lactate assay using a centrifugal analyser and 3-acetylpyridine-adenine dinucleotide.

Authors:  M P Ewen; A R Henderson
Journal:  J Clin Pathol       Date:  1977-06       Impact factor: 3.411

3.  Activation of p38 in C2C12 myotubes following ATP depletion depends on extracellular glucose.

Authors:  Chia George Hsu; Thomas J Burkholder
Journal:  J Physiol Biochem       Date:  2015-04-04       Impact factor: 4.158

4.  Argininosuccinate synthetase regulates hepatic AMPK linking protein catabolism and ureagenesis to hepatic lipid metabolism.

Authors:  Anila K Madiraju; Tiago Alves; Xiaojian Zhao; Gary W Cline; Dongyan Zhang; Sanjay Bhanot; Varman T Samuel; Richard G Kibbey; Gerald I Shulman
Journal:  Proc Natl Acad Sci U S A       Date:  2016-05-31       Impact factor: 11.205

5.  Structural analyses to identify selective inhibitors of glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme.

Authors:  Polina V Danshina; Weidong Qu; Brenda R Temple; Rafael J Rojas; Michael J Miley; Mischa Machius; Laurie Betts; Deborah A O'Brien
Journal:  Mol Hum Reprod       Date:  2016-02-26       Impact factor: 4.025

6.  The Founder Strains of the Collaborative Cross Express a Complex Combination of Advantageous and Deleterious Traits for Male Reproduction.

Authors:  Fanny Odet; Wenqi Pan; Timothy A Bell; Summer G Goodson; Alicia M Stevans; Zianing Yun; David L Aylor; Chia-Yu Kao; Leonard McMillan; Fernando Pardo-Manuel de Villena; Deborah A O'Brien
Journal:  G3 (Bethesda)       Date:  2015-10-19       Impact factor: 3.154

7.  Thin Film Growth of a Charge Transfer Cocrystal (DCS/TFPA) for Ambipolar Thin Film Transistors.

Authors:  Wolfgang Rao Bodlos; Sang Kyu Park; Birgit Kunert; Soo Young Park; Roland Resel
Journal:  ACS Appl Electron Mater       Date:  2021-06-02
  7 in total

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