BACKGROUND: One of the most frequent malignancies in women worldwide is carcinoma of the uterine cervix. High-risk human papillomavirus (HPV) infection is considered the most important etiological factor of uterine cervical cancer. Our aim was to identify novel cellular genes that could potentially act as predictive molecular markers for human cervical cancer by means of cDNA arrays. METHODS: We used cDNA arrays to examine the expression profiles of six cell lines derived from human cervical cancer, three HPV+ tumor samples and three normal (HPV-) epithelium tissues. Data normalization was performed and the top overexpressed genes were obtained. Hierarchical cluster was performed and, to validate some of the differentially expressed genes between normal and carcinogenic samples, semi-quantitative RT-PCR, in situ hybridization and immunohistochemistry were performed in tissue samples. RESULTS: Four genes were demonstrated to be consistently overexpressed in invasive cervical cancer biopsies; three novel genes not previously related to cervical cancer: MMP10, Lamc2 and Claudin 1. Moreover, overexpression of IL6 and VEGF was corroborated. CONCLUSIONS: The identification of characteristic molecular changes in cervical cells by carcinogenesis and HPV infection can lead to a better understanding of cervical cancer. cDNA arrays are beginning to provide new possible molecular markers for prognosis and diagnosis. This technology could eventually help to elucidate the biological differences of the particular mechanisms associated with each different HPV-type infection and those with a poor prognosis.
BACKGROUND: One of the most frequent malignancies in women worldwide is carcinoma of the uterine cervix. High-risk humanpapillomavirus (HPV) infection is considered the most important etiological factor of uterine cervical cancer. Our aim was to identify novel cellular genes that could potentially act as predictive molecular markers for humancervical cancer by means of cDNA arrays. METHODS: We used cDNA arrays to examine the expression profiles of six cell lines derived from humancervical cancer, three HPV+ tumor samples and three normal (HPV-) epithelium tissues. Data normalization was performed and the top overexpressed genes were obtained. Hierarchical cluster was performed and, to validate some of the differentially expressed genes between normal and carcinogenic samples, semi-quantitative RT-PCR, in situ hybridization and immunohistochemistry were performed in tissue samples. RESULTS: Four genes were demonstrated to be consistently overexpressed in invasive cervical cancer biopsies; three novel genes not previously related to cervical cancer: MMP10, Lamc2 and Claudin 1. Moreover, overexpression of IL6 and VEGF was corroborated. CONCLUSIONS: The identification of characteristic molecular changes in cervical cells by carcinogenesis and HPV infection can lead to a better understanding of cervical cancer. cDNA arrays are beginning to provide new possible molecular markers for prognosis and diagnosis. This technology could eventually help to elucidate the biological differences of the particular mechanisms associated with each different HPV-type infection and those with a poor prognosis.
Authors: Márta Benczik; Ádám Galamb; Róbert Koiss; Attila Kovács; Balázs Járay; Tamás Székely; Tímea Szekerczés; Zsuzsa Schaff; Gábor Sobel; Csaba Jeney Journal: Pathol Oncol Res Date: 2016-01 Impact factor: 3.201
Authors: T Hagemann; T Bozanovic; S Hooper; A Ljubic; V I F Slettenaar; J L Wilson; N Singh; S A Gayther; J H Shepherd; P O A Van Trappen Journal: Br J Cancer Date: 2007-01-29 Impact factor: 7.640
Authors: Agnieszka Szala; Sambor Sawicki; Anna St Swierzko; Janusz Szemraj; Marcin Sniadecki; Mateusz Michalski; Andrzej Kaluzynski; Jolanta Lukasiewicz; Anna Maciejewska; Dariusz Wydra; David C Kilpatrick; Misao Matsushita; Maciej Cedzynski Journal: Cancer Immunol Immunother Date: 2013-06-07 Impact factor: 6.968
Authors: Daniel Marrero-Rodríguez; Keiko Taniguchi-Ponciano; Malayannan Subramaniam; John R Hawse; Kevin S Pitel; Hugo Arreola-De la Cruz; Victor Huerta-Padilla; Gustavo Ponce-Navarrete; Ma Del Pilar Figueroa-Corona; Laura Gomez-Virgilio; Teresa I Martinez-Cuevas; Monica Mendoza-Rodriguez; Miriam Rodriguez-Esquivel; Pablo Romero-Morelos; Jorge Ramirez-Salcedo; Michael Baudis; Marco Meraz-Rios; Florinda Jimenez-Vega; Mauricio Salcedo Journal: Sci Rep Date: 2018-06-21 Impact factor: 4.379