OBJECTIVES: Platelet release of alpha granule-derived CXC chemokines and dense granule-derived serotonin in plasma samples was evaluated. METHODS: Concentrations of the CXC chemokines beta-TG and PF4 were assayed by an enzyme immunoassay; serotonin was measured by an HPLC method. RESULTS: Beta-TG and PF4 were more easily released than serotonin by in vitro procedures. Use of the anti-platelet cocktail CTAD and preservation of the samples at 4 degrees C were necessary to accurately measure beta-TG and PF4, but not serotonin. CONCLUSIONS: Assaying serotonin may be useful for assessing platelet activation in vivo as a laboratory test because of facile preparation of plasma samples.
OBJECTIVES: Platelet release of alpha granule-derived CXC chemokines and dense granule-derived serotonin in plasma samples was evaluated. METHODS: Concentrations of the CXC chemokines beta-TG and PF4 were assayed by an enzyme immunoassay; serotonin was measured by an HPLC method. RESULTS:Beta-TG and PF4 were more easily released than serotonin by in vitro procedures. Use of the anti-platelet cocktail CTAD and preservation of the samples at 4 degrees C were necessary to accurately measure beta-TG and PF4, but not serotonin. CONCLUSIONS: Assaying serotonin may be useful for assessing platelet activation in vivo as a laboratory test because of facile preparation of plasma samples.
Authors: Sara C Torfs; An A Maes; Catherine J Delesalle; Bart Pardon; Siska M Croubels; Piet Deprez Journal: Can Vet J Date: 2015-02 Impact factor: 1.008
Authors: S Misiti; A Stigliano; M Borro; G Gentile; S Michienzi; L Cerquetti; B Bucci; N Argese; E Brunetti; M Simmaco; V Toscano Journal: J Endocrinol Invest Date: 2009-10-09 Impact factor: 4.256