Literature DB >> 16094970

In vitro synthesis of enzymes of the tryptophan operon of Escherichia coli.

P H Pouwels1, J van Rotterdam.   

Abstract

In vitro synthesis of enzymes of the tryptophan (trp) operon of E. coli was studied in an extract prepared from E. coli, which is programmed with purified DNA from trp transducing phages with mutations that effect the expression of the trp genes in various ways. Our results show that control of transcription, i.e. initiation and termination, is similar in vivo and in vitro.

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Year:  1975        PMID： 16094970     DOI： 10.1007/bf00334014

Source DB:  PubMed          Journal:  Mol Gen Genet        ISSN： 0026-8925


  26 in total

1.  The trpE gene of Escherichia coli K contains a recognition sequence for the K-restriction system.

Authors:  N E Murray; W J Brammar
Journal:  J Mol Biol       Date:  1973-07-15       Impact factor: 5.469

2.  Thr region between the operator and first structural gene of the tryptophan operon of Escherichia coli may have a regulatory function.

Authors:  E N Jackson; C Yanofsky
Journal:  J Mol Biol       Date:  1973-05-05       Impact factor: 5.469

3.  The internal low-efficiency promoter of the tryptophan operon of Escherichia coli.

Authors:  D E Morse; C Yanofsky
Journal:  J Mol Biol       Date:  1968-12       Impact factor: 5.469

4.  Expression of the trp operon in phi80trp transducing phages. Orientation of transcription and an artificial high-efficiency promotor in phage lambda h +phi80 pt5-2AB.

Authors:  P H Pouwels; W F Stevens
Journal:  Mol Gen Genet       Date:  1973-01-18

5.  Studies of novel transducing variants of lambda: dispensability of genes N and Q.

Authors:  D Court; K Sato
Journal:  Virology       Date:  1969-10       Impact factor: 3.616

6.  Controls of rightward transcription in coliphage lambda.

Authors:  H J Nijkamp; K Bovre; W Szybalski
Journal:  J Mol Biol       Date:  1970-12-28       Impact factor: 5.469

7.  Orientation and control of transcription in E. coli phage lambda.

Authors:  S Kumar; K Bovre; A Guha; Z Hradecna; V R Maher; W Szybalski
Journal:  Nature       Date:  1969-03-01       Impact factor: 49.962

8.  Gene expression in vitro from deoxyribonucleic acid of bacteriophage T7.

Authors:  M Schweiger; P Herrlich; R L Millette
Journal:  J Biol Chem       Date:  1971-11-25       Impact factor: 5.157

9.  Positive control of endolysin synthesis in vitro by the gene N protein of phage lambda.

Authors:  J Greenblatt
Journal:  Proc Natl Acad Sci U S A       Date:  1972-12       Impact factor: 11.205

10.  Release of polarity in Escherichia coli by gene N of phage lambda: termination and antitermination of transcription.

Authors:  S Adhya; M Gottesman; B De Crombrugghe
Journal:  Proc Natl Acad Sci U S A       Date:  1974-06       Impact factor: 11.205
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  4 in total

1.  The role of IF-3 in the translation of T7- and phi80trp messenger RNA.

Authors:  R Benne; P H Pouwels
Journal:  Mol Gen Genet       Date:  1975-09-08

2.  In vitro synthesis of enzymes of the tryptophan operon of Escherichia coli. Evidence for positive control of transcription.

Authors:  P H Pouwels; J van Rotterdam
Journal:  Mol Gen Genet       Date:  1975

3.  Punctuation of transcription in vitro of the tryptophan operon of Escherichia coli. A novel type of control of transcription.

Authors:  H Pannekoek; W J Brammar; P H Pouwels
Journal:  Mol Gen Genet       Date:  1975

4.  A transcriptional barrier in the regulatory region of the tryptophan operon of Escherichia coli: its role in the regulation of repressor-independent RNA synthesis.

Authors:  P H Pouwels; H Pannekoek
Journal:  Mol Gen Genet       Date:  1976-12-22
  4 in total

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