Rui-Hai Chu1, Li-Xian Ma, Gang Wang, Li-Hua Shao. 1. Department of Infectious Diseases, Qilu Hospital of Shandong University, Jinan, Shandong Province, China. rhchu@163.com
Abstract
AIM: To investigate the influence of HLA-DRB(1) alleles and HBV genotypes on interferon-alpha therapy for chronic hepatitis B. METHODS: HLA-DRB1*03, *07, *09, *12, *15 alleles were determined using polymerase chain reaction/sequence specific primer (PCR/SSP) technique in 126 patients with chronic hepatitis B and 76 normal control subjects in Shandong Province, and HBV genotypes were determined by nested-PCR analysis using type-specific primers in 126 patients. RESULTS: The positivity of HLA-DRB1*07 allele in chronic hepatitis B group was significantly higher than that in normal control group (chi(2) = 6.33, P<0.025, RR = 2.37). Among the 126 patients, genotype B was found in 38 (30.2%), genotype C in 69 (54.8%), and mixed genotype (B+C) in 19 (15.0%), genotypes D-F were not found. Among the 46 DRB1*07(+) patients, 7 were responders and 39 were non-responders among them (chi(2) = 6.71, P<0.05). The positivity of HLA-DRB1*07 and prevalence of HBV genotype C were significantly higher in non-responders than in responders. CONCLUSION: High positivities of HLA-DRB1 *07 allele and HBV genotype C are closely associated with the lower response to interferon-alpha therapy for chronic hepatitis B.
AIM: To investigate the influence of HLA-DRB(1) alleles and HBV genotypes on interferon-alpha therapy for chronic hepatitis B. METHODS:HLA-DRB1*03, *07, *09, *12, *15 alleles were determined using polymerase chain reaction/sequence specific primer (PCR/SSP) technique in 126 patients with chronic hepatitis B and 76 normal control subjects in Shandong Province, and HBV genotypes were determined by nested-PCR analysis using type-specific primers in 126 patients. RESULTS: The positivity of HLA-DRB1*07 allele in chronic hepatitis B group was significantly higher than that in normal control group (chi(2) = 6.33, P<0.025, RR = 2.37). Among the 126 patients, genotype B was found in 38 (30.2%), genotype C in 69 (54.8%), and mixed genotype (B+C) in 19 (15.0%), genotypes D-F were not found. Among the 46 DRB1*07(+) patients, 7 were responders and 39 were non-responders among them (chi(2) = 6.71, P<0.05). The positivity of HLA-DRB1*07 and prevalence of HBV genotype C were significantly higher in non-responders than in responders. CONCLUSION: High positivities of HLA-DRB1 *07 allele and HBV genotype C are closely associated with the lower response to interferon-alpha therapy for chronic hepatitis B.
Authors: I Tatulli; R Francavilla; G L Rizzo; V Vinciguerra; E Ierardi; A Amoruso; C Panella; A Francavilla Journal: J Hepatol Date: 2001-12 Impact factor: 25.083
Authors: P D Swenson; C Van Geyt; E R Alexander; H Hagan; J M Freitag-Koontz; S Wilson; H Norder; L O Magnius; L Stuyver Journal: J Med Virol Date: 2001-07 Impact factor: 2.327
Authors: L Blitz; F H Pujol; P D Swenson; L Porto; R Atencio; M Araujo; L Costa; D C Monsalve; J R Torres; H A Fields; S Lambert; C Van Geyt; H Norder; L O Magnius; J M Echevarría; L Stuyver Journal: J Clin Microbiol Date: 1998-03 Impact factor: 5.948