Dong Li1, Su-Wen Zhu, Dong-Juan Liu, Guo-Liang Liu. 1. Department of Infectious Disease, the First Affiliated Hospital of China Medical University, Shenyang, Liaoning Province, China. ldcmu@yahoo.com.cn
Abstract
AIM: To investigate the expression of interferon inducible protein-10 (IP-10) in pancreas of mice and to discuss its possible role in the pathogenesis of type 1 diabetes. METHODS: Non-obese diabetic (NOD) mice were used as experiment group and BALB/c mice as non-diabetic prone model. Immunohistochemistry method was used to evaluate the expression of IP-10 in the pancreas of NOD mice and BALB/c mice. Immunoelectron microscope was used to show the location of IP-10 in pancreatic islet beta cells. RESULTS: Pancreatic islets were positively stained in all the NOD mice. Insulitis could be found in mice at the age of 4 wk. The weakly positive results were found in control group with no insulitis. Immunoelectron microscopy further demonstrated that IP-10 was produced by pancreatic beta cells and stored in cytoplasm of the cells. CONCLUSION: IP-10 can be largely produced in pancreatic islets of NOD mice at the age of 2 wk when there is no significant insulitis, and may play an important part in the pathogenesis of type 1 diabetes by attracting immune cells to infiltrate the pancreatic islets.
AIM: To investigate the expression of interferon inducible protein-10 (IP-10) in pancreas of mice and to discuss its possible role in the pathogenesis of type 1 diabetes. METHODS:Non-obese diabetic (NOD) mice were used as experiment group and BALB/c mice as non-diabetic prone model. Immunohistochemistry method was used to evaluate the expression of IP-10 in the pancreas of NOD mice and BALB/c mice. Immunoelectron microscope was used to show the location of IP-10 in pancreatic islet beta cells. RESULTS:Pancreatic islets were positively stained in all the NOD mice. Insulitis could be found in mice at the age of 4 wk. The weakly positive results were found in control group with no insulitis. Immunoelectron microscopy further demonstrated that IP-10 was produced by pancreatic beta cells and stored in cytoplasm of the cells. CONCLUSION:IP-10 can be largely produced in pancreatic islets of NOD mice at the age of 2 wk when there is no significant insulitis, and may play an important part in the pathogenesis of type 1 diabetes by attracting immune cells to infiltrate the pancreatic islets.
Authors: L M Bradley; V C Asensio; L K Schioetz; J Harbertson; T Krahl; G Patstone; N Woolf; I L Campbell; N Sarvetnick Journal: J Immunol Date: 1999-03-01 Impact factor: 5.422
Authors: A Shimada; J Morimoto; K Kodama; R Suzuki; Y Oikawa; O Funae; A Kasuga; T Saruta; S Narumi Journal: Diabetes Care Date: 2001-03 Impact factor: 19.112
Authors: Naureen Javeed; Tracy K Her; Matthew R Brown; Patrick Vanderboom; Kuntol Rakshit; Aoife M Egan; Adrian Vella; Ian Lanza; Aleksey V Matveyenko Journal: Cell Rep Date: 2021-08-24 Impact factor: 9.423