| Literature DB >> 1609057 |
A A Seiliev1, N B Zvonareva, B D Zhivotovsky, K P Hanson.
Abstract
The spectrum of nuclear nucleases in control and irradiated (4 Gy) thymocytes has been investigated. Using the method of SDS electrophoresis of nuclear proteins in 3H-DNA-polyacrylamide gels a number of polypeptides of MW. 35, 32, 17.7, 17.2 and 16.4 kDa possessing nuclease activity were found. The 35 kDa enzyme is only active in the presence of Ca2+ and Mg2+ ions. In response to cycloheximide injection (3 mg/100 g body weight) and irradiation, we did not detect the 35 kDa nuclease activity. Nucleases of 32, 17.7, 17.2 and 16.4 kDa are active in the presence of Ca2+ ions. The activities of these nucleases increases 60 min after irradiation. These nucleases were also found in the fraction of polydeoxyribonucleotide (PDN).Entities:
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Year: 1992 PMID： 1609057 DOI： 10.1007/bf01211210
Source DB: PubMed Journal: Radiat Environ Biophys ISSN： 0301-634X Impact factor: 1.925