PURPOSE: To report positive polymerase chain reaction (PCR) for Mycobacterium tuberculosis from the vitreous and epididymal fluid in a patient of choroidal tuberculoma and epididymitis. DESIGN: Interventional case report. SETTING: Institutional setting. intervention: Vitreous and epididymal fluid tap subjected to PCR for primer IS 6110 sequence specific for M. tuberculosis. Purification of DNA product and sequencing on an automated DNA sequencer. Institution of antitubercular therapy. MAIN OUTCOME MEASURE: Matching of sequence with NCBI's data bank response to antitubercular therapy. RESULTS: DNA products from vitreous and epidiymal fluid matched with IS 6110 sequence of M. tuberculosis. Choroidal granuloma and epididymitis responded to antitubercular therapy. CONCLUSIONS: Simultaneous choroidal tuberculoma and epididymitis resulted from an infection by M. tuberculosis, and both matched with IS 6110 sequence of M. tuberculosis.
PURPOSE: To report positive polymerase chain reaction (PCR) for Mycobacterium tuberculosis from the vitreous and epididymal fluid in a patient of choroidal tuberculoma and epididymitis. DESIGN: Interventional case report. SETTING: Institutional setting. intervention: Vitreous and epididymal fluid tap subjected to PCR for primer IS 6110 sequence specific for M. tuberculosis. Purification of DNA product and sequencing on an automated DNA sequencer. Institution of antitubercular therapy. MAIN OUTCOME MEASURE: Matching of sequence with NCBI's data bank response to antitubercular therapy. RESULTS: DNA products from vitreous and epidiymal fluid matched with IS 6110 sequence of M. tuberculosis. Choroidal granuloma and epididymitis responded to antitubercular therapy. CONCLUSIONS: Simultaneous choroidal tuberculoma and epididymitis resulted from an infection by M. tuberculosis, and both matched with IS 6110 sequence of M. tuberculosis.