| Literature DB >> 16084586 |
C S Cho1, S J Seo, I K Park, S H Kim, T H Kim, T Hoshiba, I Harada, T Akaike.
Abstract
Extracellular matrix (ECM) plays important roles in tissue engineering because cellular growth and differentiation, in the two-dimensional cell culture as well as in the three-dimensional space of the developing organism, require ECM with which the cells can interact. Especially, the bioartificial liver-assist device or regeneration of the liver-tissue substitutes for liver tissue engineering requires a suitable ECM for hepatocyte culture because hepatocytes are anchorage-dependent cells and are highly sensitive to the ECM milieu for the maintenance of their viability and differentiated functions. Galactose-carrying synthetic ECMs derived from synthetic polymers and natural polymers bind hepatocytes through a receptor-mediated mechanism, resulting in enhanced hepatocyte functions. Attachment and functions of hepatocytes were affected by physico-chemical properties including ECM geometry as well as the type, density and orientation of galactose. Also, cellular environment, medium composition and dynamic culture system influenced liver-specific functions of hepatocytes beside ECM.Entities:
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Year: 2005 PMID： 16084586 DOI： 10.1016/j.biomaterials.2005.06.008
Source DB: PubMed Journal: Biomaterials ISSN： 0142-9612 Impact factor: 12.479