AIMS: Matrix metalloproteinase (MMP) activity is increased after radiation. The aims of this study were to assess the time course of this increase and its effects on malignant cell invasion. METHODS: Colorectal cancer (HCT 116, LoVo, C 170 HM 2, CaCO-2), fibroblast (46-BR.IGI, CCD-18 Co) and fibrosarcoma (HT1080) cell lines were irradiated at 4 gray (4 Gy) and matrix metalloproteinase gene and protein expression examined over a 96 h period by real time polymerase chain reaction and gelatin zymography. Invasion was assessed on Matrigel. Human rectal tumour MMP expression was compared before and after long course radiotherapy. RESULTS: Radiation increased MMP gene expression of tumour cell lines, and resulted in increased MMP protein activity in the HT1080 line. HT1080 and HCT 116 in monoculture and LoVo in co-culture were more invasive after radiation at 48 h in vitro, but long course radiotherapy did not result in a consistent increase in MMP expression from human rectal tumour biopsies. CONCLUSIONS: Radiation results in increased MMP expression for a limited time period. This results in an early increase in cell line invasion. Further clinical research is required to clarify if MMP inhibition given perioperatively following radiotherapy decreases local recurrence rates.
AIMS: Matrix metalloproteinase (MMP) activity is increased after radiation. The aims of this study were to assess the time course of this increase and its effects on malignant cell invasion. METHODS:Colorectal cancer (HCT 116, LoVo, C 170 HM 2, CaCO-2), fibroblast (46-BR.IGI, CCD-18 Co) and fibrosarcoma (HT1080) cell lines were irradiated at 4 gray (4 Gy) and matrix metalloproteinase gene and protein expression examined over a 96 h period by real time polymerase chain reaction and gelatin zymography. Invasion was assessed on Matrigel. Human rectal tumour MMP expression was compared before and after long course radiotherapy. RESULTS: Radiation increased MMP gene expression of tumour cell lines, and resulted in increased MMP protein activity in the HT1080 line. HT1080 and HCT 116 in monoculture and LoVo in co-culture were more invasive after radiation at 48 h in vitro, but long course radiotherapy did not result in a consistent increase in MMP expression from human rectal tumour biopsies. CONCLUSIONS: Radiation results in increased MMP expression for a limited time period. This results in an early increase in cell line invasion. Further clinical research is required to clarify if MMP inhibition given perioperatively following radiotherapy decreases local recurrence rates.
Authors: Odysseas Kargiotis; Chandramu Chetty; Venkateswara Gogineni; Christopher S Gondi; Sai Muralikrishna Pulukuri; Athanassios P Kyritsis; Meena Gujrati; Jeffrey D Klopfenstein; Dzung H Dinh; Jasti S Rao Journal: Int J Oncol Date: 2008-11 Impact factor: 5.650
Authors: Ana Cristina de Albuquerque-Xavier; Lilian Gonçalves R Bastos; Julio Cesar Madureira de Freitas; Fernanda Leve; Waldemir Fernández de Souza; Wallace Martins de Araujo; João Luiz Mendes Wanderley; Marcelo Neves Tanaka; Wanderley de Souza; José Andrés Morgado-Díaz Journal: Int J Oncol Date: 2012-01-10 Impact factor: 5.650
Authors: Lisa Buckel; Elamprakash N Savariar; Jessica L Crisp; Karra A Jones; Angel M Hicks; Daniel J Scanderbeg; Quyen T Nguyen; Jason K Sicklick; Andrew M Lowy; Roger Y Tsien; Sunil J Advani Journal: Cancer Res Date: 2015-02-13 Impact factor: 12.701
Authors: Saulius Svagzdys; Vaiva Lesauskaite; Dainius Pavalkis; Irena Nedzelskiene; Darius Pranys; Algimantas Tamelis Journal: BMC Cancer Date: 2009-03-26 Impact factor: 4.430