Literature DB >> 16061963

Isolation, propagation, and titration of human immunodeficiency virus type 1 from peripheral blood of infected individuals.

Hanneke Schuitemaker1, Neeltje A Kootstra.   

Abstract

HIV-1 can be isolated from peripheral blood mononuclear cells and is easily propagated on primary cells in vitro. Here we describe the method for bulk isolation of the HIV-1 quasispecies and a limiting dilution virus isolation protocol by which single coexisting clones can be obtained. In addition, methods for propagation and titration of HIV-1 are provided.

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Year:  2005        PMID： 16061963     DOI： 10.1385/1-59259-907-9:017

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


  7 in total

1.  Neutralization potential of the plasma of HIV-1 infected Indian patients in the context of anti-V3 antibody content and antiretroviral therapy. [corrected].

Authors:  Alok Kumar Choudhary; Raiees Andrabi; Somi Sankaran Prakash; Rajesh Kumar; Shubhasree Dutta Choudhury; Naveet Wig; Ashutosh Biswas; Anjali Hazarika; Kalpana Luthra
Journal:  J Microbiol       Date:  2012-02-27       Impact factor: 3.422

2.  Primary isolates of human immunodeficiency virus type 1 are usually dominated by the major variants found in blood.

Authors:  Yegor Voronin; Bhavna Chohan; Michael Emerman; Julie Overbaugh
Journal:  J Virol       Date:  2007-07-25       Impact factor: 5.103

3.  Toll-like receptor-3 mediates HIV-1 transactivation via NFκB and JNK pathways and histone acetylation, but prolonged activation suppresses Tat and HIV-1 replication.

Authors:  Biju Bhargavan; Shawna M Woollard; Georgette D Kanmogne
Journal:  Cell Signal       Date:  2015-11-11       Impact factor: 4.315

4.  Data in support of NFκB and JNK pathways involvement in TLR3-mediated HIV-1 transactivation, expression of IL-6 and transcription factors associated with HIV-1 replication.

Authors:  Biju Bhargavan; Shawna M Woollard; Georgette D Kanmogne
Journal:  Data Brief       Date:  2015-12-17

5.  Epigenetics, N-myrystoyltransferase-1 and casein kinase-2-alpha modulates the increased replication of HIV-1 CRF02_AG, compared to subtype-B viruses.

Authors:  Biju Bhargavan; Georgette D Kanmogne
Journal:  Sci Rep       Date:  2019-07-23       Impact factor: 4.379

6.  Commentary: Questioning the HIV-AIDS Hypothesis: 30 Years of Dissent.

Authors:  Alexey Karetnikov
Journal:  Front Public Health       Date:  2015-08-07

7.  Effects of Cigarette Smoke Condensate on Oxidative Stress, Apoptotic Cell Death, and HIV Replication in Human Monocytic Cells.

Authors:  Pss Rao; Anusha Ande; Namita Sinha; Anil Kumar; Santosh Kumar
Journal:  PLoS One       Date:  2016-05-20       Impact factor: 3.240
  7 in total

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