OBJECTIVE: The potential relationship between adenosine deaminase activity and cancer progression was examined by investigating the activity of total ADA and its isoenzymes in serum and simultaneously in the cancerous tissue of each patient with breast cancer. METHODS: Total ADA and its isoenzymes were measured using the Giusti method. ADA2 activity was measured in the presence of a specific ADA1 inhibitor, EHNA. RESULTS: Our results indicated that ADA2 and total ADA activities were higher in serum and malignant tissues than those of corresponding controls (P < 0.05). Tumor ADA2 and total ADA activities were significantly (P < 0.05) correlated with lymph node involvement, histological grade and tumor size, whereas their levels in serum were significantly (P < 0.05) correlated with menopausal status and patient age. CONCLUSION: Although serum and tumor total ADA activity and its ADA2 isoenzyme were both found to be increased, distinct correlation patterns were observed with some of the prognostic factors. It can be speculated that increased ADA and isoenzyme activities in serum originated from sources other than the breast tumors.
OBJECTIVE: The potential relationship between adenosine deaminase activity and cancer progression was examined by investigating the activity of total ADA and its isoenzymes in serum and simultaneously in the cancerous tissue of each patient with breast cancer. METHODS: Total ADA and its isoenzymes were measured using the Giusti method. ADA2 activity was measured in the presence of a specific ADA1 inhibitor, EHNA. RESULTS: Our results indicated that ADA2 and total ADA activities were higher in serum and malignant tissues than those of corresponding controls (P < 0.05). TumorADA2 and total ADA activities were significantly (P < 0.05) correlated with lymph node involvement, histological grade and tumor size, whereas their levels in serum were significantly (P < 0.05) correlated with menopausal status and patient age. CONCLUSION: Although serum and tumor total ADA activity and its ADA2 isoenzyme were both found to be increased, distinct correlation patterns were observed with some of the prognostic factors. It can be speculated that increased ADA and isoenzyme activities in serum originated from sources other than the breast tumors.
Authors: Jaehyun Kim; Karl-Erik Andersson; John D Jackson; Sang Jin Lee; Anthony Atala; James J Yoo Journal: Tissue Eng Part A Date: 2014-07-02 Impact factor: 3.845
Authors: Daniela Zanini; Roberta Schmatz; Luana Paula Pelinson; Victor Camera Pimentel; Pauline da Costa; Andréia Machado Cardoso; Caroline Curry Martins; Christina Chitolina Schetinger; Jucimara Baldissareli; Maria do Carmo Araújo; Liliane Oliveira; Juarez Chiesa; Vera Maria Morsch; Daniela Bitencourt Rosa Leal; Maria Rosa Chitolina Schetinger Journal: Mol Cell Biochem Date: 2012-11-18 Impact factor: 3.396
Authors: Siqi Chen; Derek A Wainwright; Jennifer D Wu; Yong Wan; Daniela E Matei; Yi Zhang; Bin Zhang Journal: Immunotherapy Date: 2019-06-21 Impact factor: 4.196