Yu Fan1, Shu Zheng, Ze-Feng Xu, Jia-Yi Ding. 1. Cancer Institute, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou 310009, Zhejiang Province, China.
Abstract
AIM: To investigate the effects of polo-like kinase-1 (PLK1) antisense phosphorothioate oligodeoxynucleotide (ASODN) on apoptosis and cell cycle of human colon cancer cell line SW480. METHODS: After SW480 colon cancer cells were transfected with PLK1 ASODN, Northern and Western blot analyses were used to examine PLK1 gene expression in cancer cells. We studied apoptosis using terminal uridine deoxynucleotidyl nick end labeling. Apoptosis and cell cycle of SW480 cells were examined by fluorescence-activated cell sorter scan. RESULTS: The levels of PLK1 mRNA and protein were greatly inhibited by PLK1 ASODN in SW480 cancer cells transfected with PLK1 ASODN. Apoptosis index (AI) induced PLK1 ASODN in a time- and dose-dependent manner. Results from FLM showed that sub-2N DNA content of transfected cancer cells was significantly increased and arrested at G2/M compared with control groups. CONCLUSION: PLK1 ASODN can induce apoptosis of human colon cancer cell line SW480.
AIM: To investigate the effects of polo-like kinase-1 (PLK1) antisense phosphorothioateoligodeoxynucleotide (ASODN) on apoptosis and cell cycle of humancolon cancer cell line SW480. METHODS: After SW480 colon cancer cells were transfected with PLK1ASODN, Northern and Western blot analyses were used to examine PLK1 gene expression in cancer cells. We studied apoptosis using terminal uridine deoxynucleotidyl nick end labeling. Apoptosis and cell cycle of SW480 cells were examined by fluorescence-activated cell sorter scan. RESULTS: The levels of PLK1 mRNA and protein were greatly inhibited by PLK1ASODN in SW480 cancer cells transfected with PLK1ASODN. Apoptosis index (AI) induced PLK1ASODN in a time- and dose-dependent manner. Results from FLM showed that sub-2N DNA content of transfected cancer cells was significantly increased and arrested at G2/M compared with control groups. CONCLUSION:PLK1ASODN can induce apoptosis of humancolon cancer cell line SW480.
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