BACKGROUND: Chronic fatigue syndrome (CFS) is a multisystem disease, the pathogenesis of which remains undetermined. AIMS: To test the hypothesis that there are reproducible abnormalities of gene expression in patients with CFS compared with normal healthy persons. METHODS: To gain further insight into the pathogenesis of this disease, gene expression was analysed in peripheral blood mononuclear cells from 25 patients with CFS diagnosed according to the Centers for Disease Control criteria and 25 normal blood donors matched for age, sex, and geographical location, using a single colour microarray representing 9522 human genes. After normalisation, average difference values for each gene were compared between test and control groups using a cutoff fold difference of expression > or = 1.5 and a p value of 0.001. Genes showing differential expression were further analysed using Taqman real time polymerase chain reaction (PCR) in fresh samples. RESULTS: Analysis of microarray data revealed differential expression of 35 genes. Real time PCR confirmed differential expression in the same direction as array results for 16 of these genes, 15 of which were upregulated (ABCD4, PRKCL1, MRPL23, CD2BP2, GSN, NTE, POLR2G, PEX16, EIF2B4, EIF4G1, ANAPC11, PDCD2, KHSRP, BRMS1, and GABARAPL1) and one of which was downregulated (IL-10RA). This profile suggests T cell activation and perturbation of neuronal and mitochondrial function. Upregulation of neuropathy target esterase and eukaryotic translation initiation factor 4G1 may suggest links with organophosphate exposure and virus infection, respectively. CONCLUSION: These results suggest that patients with CFS have reproducible alterations in gene regulation.
BACKGROUND: Chronic fatigue syndrome (CFS) is a multisystem disease, the pathogenesis of which remains undetermined. AIMS: To test the hypothesis that there are reproducible abnormalities of gene expression in patients with CFS compared with normal healthy persons. METHODS: To gain further insight into the pathogenesis of this disease, gene expression was analysed in peripheral blood mononuclear cells from 25 patients with CFS diagnosed according to the Centers for Disease Control criteria and 25 normal blood donors matched for age, sex, and geographical location, using a single colour microarray representing 9522 human genes. After normalisation, average difference values for each gene were compared between test and control groups using a cutoff fold difference of expression > or = 1.5 and a p value of 0.001. Genes showing differential expression were further analysed using Taqman real time polymerase chain reaction (PCR) in fresh samples. RESULTS: Analysis of microarray data revealed differential expression of 35 genes. Real time PCR confirmed differential expression in the same direction as array results for 16 of these genes, 15 of which were upregulated (ABCD4, PRKCL1, MRPL23, CD2BP2, GSN, NTE, POLR2G, PEX16, EIF2B4, EIF4G1, ANAPC11, PDCD2, KHSRP, BRMS1, and GABARAPL1) and one of which was downregulated (IL-10RA). This profile suggests T cell activation and perturbation of neuronal and mitochondrial function. Upregulation of neuropathy target esterase and eukaryotic translation initiation factor 4G1 may suggest links with organophosphate exposure and virus infection, respectively. CONCLUSION: These results suggest that patients with CFS have reproducible alterations in gene regulation.
Authors: Emile F Nuwaysir; Wei Huang; Thomas J Albert; Jaz Singh; Kate Nuwaysir; Alan Pitas; Todd Richmond; Tom Gorski; James P Berg; Jeff Ballin; Mark McCormick; Jason Norton; Tim Pollock; Terry Sumwalt; Lawrence Butcher; DeAnn Porter; Michael Molla; Christine Hall; Fred Blattner; Michael R Sussman; Rodney L Wallace; Franco Cerrina; Roland D Green Journal: Genome Res Date: 2002-11 Impact factor: 9.043
Authors: A K Rasmussen; H Nielsen; V Andersen; T Barington; K Bendtzen; M B Hansen; L Nielsen; B K Pedersen; A Wiik Journal: J Rheumatol Date: 1994-08 Impact factor: 4.666
Authors: Suzanne D Vernon; Elizabeth R Unger; Irina M Dimulescu; Mangalathu Rajeevan; William C Reeves Journal: Dis Markers Date: 2002 Impact factor: 3.434
Authors: Peter Chu; Jorge Pardo; Haoran Zhao; Connie C Li; Erlina Pali; Mary M Shen; Kunbin Qu; Simon X Yu; Betty Cb Huang; Peiwen Yu; Esteban S Masuda; Susan M Molineaux; Frank Kolbinger; Gregorio Aversa; Jan de Vries; Donald G Payan; X Charlene Liao Journal: J Biol Date: 2003-09-15
Authors: Katrine Brække Norheim; Stephanie Le Hellard; Gunnel Nordmark; Erna Harboe; Lasse Gøransson; Johan G Brun; Marie Wahren-Herlenius; Roland Jonsson; Roald Omdal Journal: Rheumatol Int Date: 2013-09-03 Impact factor: 2.631
Authors: J R Kerr; P Christian; A Hodgetts; P R Langford; L D Devanur; R Petty; B Burke; L I Sinclair; S C M Richards; J Montgomery; C R McDermott; T J Harrison; P Kellam; D J Nutt; S T Holgate Journal: J Clin Pathol Date: 2006-08-25 Impact factor: 3.411
Authors: Natalya Frolova; Mick D Edmonds; Thomas M Bodenstine; Robert Seitz; Martin R Johnson; Rui Feng; Danny R Welch; Andra R Frost Journal: Tumour Biol Date: 2009-07-16
Authors: John W Gow; Suzanne Hagan; Pawel Herzyk; Celia Cannon; Peter O Behan; Abhijit Chaudhuri Journal: BMC Med Genomics Date: 2009-06-25 Impact factor: 3.063