Jan Styczynski1, Mariusz Wysocki. 1. Department of Pediatric Hematology and Oncology, Collegium Medicum of Bydgoszcz, Nicolaus Copernicus University of Torun, Bydgoszcz, Poland. jstyczynski@cm.umk.pl
Abstract
OBJECTIVE: Ex vivo activity of three products of alternative therapy against leukemic and normal cells was analyzed. MATERIAL AND METHODS: Extracts of Viscum album, Uncaria tomentosa, and Croton lechleri were used for the study. Leukemic cells of 53 children with acute leukemias and four cell lines, Jurkat, CCRF-CEM, HL-60, and K-562 were tested for sensitivity to alternative medicine remedies by the MTT assay, cell-cycle analysis and annexin-V binding assay. RESULTS: Leukemic cells showed high resistance to tested three compounds of alternative medicine in all performed assays. Additionally, tested remedies stimulated survival of leukemic cells in 45%, 96%, and 83% cases, respectively; while no effect was observed in normal lymphocytes. In combination studies, Viscum album extract did not increase prednisolone and cytarabine cytotoxicity in leukemic cells. CONCLUSION: We conclude that some alternative medicine remedies might stimulate the viability of childhood leukemic cells.
OBJECTIVE: Ex vivo activity of three products of alternative therapy against leukemic and normal cells was analyzed. MATERIAL AND METHODS: Extracts of Viscum album, Uncaria tomentosa, and Croton lechleri were used for the study. Leukemic cells of 53 children with acute leukemias and four cell lines, Jurkat, CCRF-CEM, HL-60, and K-562 were tested for sensitivity to alternative medicine remedies by the MTT assay, cell-cycle analysis and annexin-V binding assay. RESULTS:Leukemic cells showed high resistance to tested three compounds of alternative medicine in all performed assays. Additionally, tested remedies stimulated survival of leukemic cells in 45%, 96%, and 83% cases, respectively; while no effect was observed in normal lymphocytes. In combination studies, Viscum album extract did not increase prednisolone and cytarabinecytotoxicity in leukemic cells. CONCLUSION: We conclude that some alternative medicine remedies might stimulate the viability of childhood leukemic cells.
Authors: Ricardo Ballesteros-Ramírez; Eliana Aldana; María Victoria Herrera; Claudia Urueña; Laura Yinneth Rojas; Luis Fernando Echeverri; Geison Modesti Costa; Sandra Quijano; Susana Fiorentino Journal: Evid Based Complement Alternat Med Date: 2020-12-11 Impact factor: 2.629