Literature DB >> 16044545

Quenched fluorescent substrate-based peptidase assays.

Rebecca A Lew1, Nathalie Tochon-Danguy, Catherine A Hamilton, Karen M Stewart, Marie-Isabel Aguilar, A Ian Smith.   

Abstract

The use of specific quenched fluorescent substrates (QFS) provides a rapid and sensitive method to measure peptidase activity, and is readily adaptable to high-throughput screening of potential peptidase inhibitors. In this chapter, we discuss general considerations for the development of QFS assays, and describe in detail an assay protocol for the mammalian metallopeptidase, endothelin-converting enzyme.

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Year:  2005        PMID： 16044545     DOI： 10.1385/1-59259-877-3:143

Source DB:  PubMed          Journal:  Methods Mol Biol        ISSN： 1064-3745


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