| Literature DB >> 16044019 |
Nicolas Nagot1, Vincent Foulongne, Pierre Becquart, Philippe Mayaud, Issouf Konate, Abdoulaye Ouedraogo, Marie-Christine Defer, Helen Weiss, Philippe Van de Perre, Michel Segondy.
Abstract
Forty-two HSV-2-seropositive women, of whom 22 were HIV-1 seropositive, were followed up weekly for 3 weeks for assessment of HIV-1 and HSV-2 genital shedding in Bobo Dioulasso, Burkina Faso. HSV-2 DNA and HIV-1 RNA were quantitated in cervicovaginal lavages enriched with a cervical swabbing (eCVL) using real-time polymerase chain reaction. HIV-1 RNA shedding was detected in eCVL from 19 of 22 (86.4%) of the HIV-1-seropositive women and HSV-2 DNA was detected in eCVL from 12 of 42 (28.6%) of the women. Compared with the HIV-1-seronegative women, the HIV-1-seropositive women showed more persistent HSV-2 genital shedding and higher HSV-2 DNA loads in eCVL. This study showed that eCVL is a reliable sampling method for detecting both viruses. Three measurement points (at weekly intervals in this study) seem to adequately allow detection of most HIV-1 or HSV-2 genital shedders.Entities:
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Year: 2005 PMID： 16044019
Source DB: PubMed Journal: J Acquir Immune Defic Syndr ISSN： 1525-4135 Impact factor: 3.731