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Literature DB >> 16041055

Mutation of the zinc-binding metalloprotease motif affects Bacteroides fragilis toxin activity but does not affect propeptide processing.

Augusto A Franco¹, Simy L Buckwold, Jai W Shin, Miguel Ascon, Cynthia L Sears.

Abstract

To evaluate the role of the zinc-binding metalloprotease in Bacteroides fragilis toxin (BFT) processing and activity, the zinc-binding consensus sequences (H348, E349, H352, G355, H358, and M366) were mutated by site-directed-mutagenesis. Our results indicated that single point mutations in the zinc-binding metalloprotease motif do not affect BFT processing but do reduce or eliminate BFT biologic activity in vitro.

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Year: 2005 PMID： 16041055 PMCID： PMC1201259 DOI： 10.1128/IAI.73.8.5273-5277.2005

Source DB: PubMed Journal: Infect Immun ISSN： 0019-9567 Impact factor: 3.441

38 in total

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