Literature DB >> 16038614

A low molecular weight isoform of hyaluronidase: purification from Indian cobra (Naja naja) venom and partial characterization.

K S Girish1, K Kemparaju.   

Abstract

A low molecular weight isoform of hyaluronidase (NNH2) has been isolated from Indian cobra (Naja naja) venom by successive chromatography on Sephadex G-75 and CM-Sephadex C-25 columns. The apparent molecular weight determined by SDS-PAGE is 52 kD, and the pI value is 9.7. NNH2 is an endoglycosidase and exhibits in vitro absolute specificity for hyaluronan; it also hydrolyzed hyaluronan in human skin sections. NNH2 is nontoxic, but it indirectly potentiates the hemorrhagic activity of hemorrhagic complex-I. Curcumin, indomethacin, and tannic acid inhibited dose dependently the degradation of hyaluronan by NNH2.

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Year:  2005        PMID： 16038614     DOI： 10.1007/s10541-005-0172-6

Source DB:  PubMed          Journal:  Biochemistry (Mosc)        ISSN： 0006-2979            Impact factor:   2.487


  3 in total

1.  YsHyl8A, an Alkalophilic Cold-Adapted Glycosaminoglycan Lyase Cloned from Pathogenic Yersinia sp. 298.

Authors:  Shilong Zhang; Yujiao Li; Feng Han; Wengong Yu
Journal:  Molecules       Date:  2022-05-02       Impact factor: 4.927

2.  A novel hyaluronidase from brown spider (Loxosceles intermedia) venom (Dietrich's Hyaluronidase): from cloning to functional characterization.

Authors:  Valéria Pereira Ferrer; Thiago Lopes de Mari; Luiza Helena Gremski; Dilza Trevisan Silva; Rafael Bertoni da Silveira; Waldemiro Gremski; Olga Meiri Chaim; Andrea Senff-Ribeiro; Helena Bonciani Nader; Silvio Sanches Veiga
Journal:  PLoS Negl Trop Dis       Date:  2013-05-02

Review 3.  Brown spider (Loxosceles genus) venom toxins: tools for biological purposes.

Authors:  Olga Meiri Chaim; Dilza Trevisan-Silva; Daniele Chaves-Moreira; Ana Carolina M Wille; Valéria Pereira Ferrer; Fernando Hitomi Matsubara; Oldemir Carlos Mangili; Rafael Bertoni da Silveira; Luiza Helena Gremski; Waldemiro Gremski; Andrea Senff-Ribeiro; Silvio Sanches Veiga
Journal:  Toxins (Basel)       Date:  2011-03-22       Impact factor: 4.546
  3 in total

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