Literature DB >> 16023891

Usefulness of repeated GenomiPhi, a phi29 DNA polymerase-based rolling circle amplification kit, for generation of large amounts of plasmid DNA.

Masahiro Sato1, Masato Ohtsuka, Yuhsuke Ohmi.   

Abstract

The GenomiPhi DNA Amplification Kit employs rolling circle amplification (RCA) using phi29 polymerase, dNTPs, and random hexamers. We demonstrated that repeated RCA (at least three times) is useful for high-fidelity amplification of large amounts of plasmid DNA.

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Year:  2005        PMID： 16023891     DOI： 10.1016/j.bioeng.2005.05.001

Source DB:  PubMed          Journal:  Biomol Eng        ISSN： 1389-0344


  5 in total

1.  Evaluation of a transposase protocol for rapid generation of shotgun high-throughput sequencing libraries from nanogram quantities of DNA.

Authors:  Rachel Marine; Shawn W Polson; Jacques Ravel; Graham Hatfull; Daniel Russell; Matthew Sullivan; Fraz Syed; Michael Dumas; K Eric Wommack
Journal:  Appl Environ Microbiol       Date:  2011-09-23       Impact factor: 4.792

2.  Cell-free cloning using phi29 DNA polymerase.

Authors:  Clyde A Hutchison; Hamilton O Smith; Cynthia Pfannkoch; J Craig Venter
Journal:  Proc Natl Acad Sci U S A       Date:  2005-11-14       Impact factor: 11.205

3.  Tapping diversity lost in transformations--in vitro amplification of ligation reactions.

Authors:  Daniel Christ; Kristoffer Famm; Greg Winter
Journal:  Nucleic Acids Res       Date:  2006-08-31       Impact factor: 16.971

4.  Microbial ecology of four coral atolls in the Northern Line Islands.

Authors:  Elizabeth A Dinsdale; Olga Pantos; Steven Smriga; Robert A Edwards; Florent Angly; Linda Wegley; Mark Hatay; Dana Hall; Elysa Brown; Matthew Haynes; Lutz Krause; Enric Sala; Stuart A Sandin; Rebecca Vega Thurber; Bette L Willis; Farooq Azam; Nancy Knowlton; Forest Rohwer
Journal:  PLoS One       Date:  2008-02-27       Impact factor: 3.240

5.  Metagenomic assay for identification of microbial pathogens in tumor tissues.

Authors:  Don A Baldwin; Michael Feldman; James C Alwine; Erle S Robertson
Journal:  mBio       Date:  2014-09-16       Impact factor: 7.867
  5 in total

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