| Literature DB >> 16023641 |
L E González1, J A García, C Núñez, T M Perrone, B González-Baradat, M I Gonzatti, A Reyna-Bello.
Abstract
Trypanosoma vivax is the principal etiological agent of bovine trypanosomosis, a widely disseminated disease in tropical and subtropical regions. Here, we present a simple and reproducible method for the purification of T. vivax from experimentally infected and immunosuppressed sheep, using an isopycnic Percoll gradient, followed by DEAE-cellulose chromatography, with an estimated yield of 11-15%. This method could be used for the purification of T. vivax geographical isolates from various locations and from different natural hosts.Entities:
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Year: 2005 PMID： 16023641 DOI： 10.1016/j.exppara.2005.05.008
Source DB: PubMed Journal: Exp Parasitol ISSN： 0014-4894 Impact factor: 2.011