| Literature DB >> 16023116 |
Natalya N Samsonova1, Sergey V Smirnov, Anna E Novikova, Leonid R Ptitsyn.
Abstract
Gamma-aminobutyraldehyde dehydrogenase (ABALDH) from wild-type E. coli K12 was purified to apparent homogeneity and identified as YdcW by MS-analysis. YdcW exists as a tetramer of 202+/-29 kDa in the native state, a molecular mass of one subunit was determined as 51+/-3 kDa. Km parameters of YdcW for gamma-aminobutyraldehyde, NAD+ and NADP+ were 41+/-7, 54+/-10 and 484+/-72 microM, respectively. YdcW is the unique ABALDH in E. coli K12. A coupling action of E. coli YgjG putrescine transaminase and YdcW dehydrogenase in vitro resulted in conversion of putrescine into gamma-aminobutyric acid.Entities:
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Year: 2005 PMID: 16023116 DOI: 10.1016/j.febslet.2005.06.038
Source DB: PubMed Journal: FEBS Lett ISSN: 0014-5793 Impact factor: 4.124