PURPOSE: To study antibody production to Pseudomonas aeruginosa protease IV (PIV) for immunoassay development and to assess the possible role of antibody in arresting corneal damage. METHODS: Rabbits were immunized with PIV, urea-soluble recombinant PIV (rPIV), or precipitated rPIV. Antibody was analyzed by ELISA and Western blotting. Antibody-mediated inhibition of PIV activity was tested by colorimetric assay and during keratitis by slit-lamp examination of infected eyes. RESULTS: Antibody was not produced after PIV immunization but was induced by rPIV. Rabbits immunized first with soluble and then precipitated rPIV produced high titers (log(10)) to rPIV (4.28 +/- 0.09) and significantly higher titers to PIV (3.90 +/- 0.06) compared to the other immunized groups. Antibody to rPIV reacted with PIV, but neither neutralized enzyme activity in vitro nor protected infected rabbits in vivo. CONCLUSIONS: The present study demonstrates that PIV is a virulence factor which can escape a protective immune response.
PURPOSE: To study antibody production to Pseudomonas aeruginosa protease IV (PIV) for immunoassay development and to assess the possible role of antibody in arresting corneal damage. METHODS:Rabbits were immunized with PIV, urea-soluble recombinant PIV (rPIV), or precipitated rPIV. Antibody was analyzed by ELISA and Western blotting. Antibody-mediated inhibition of PIV activity was tested by colorimetric assay and during keratitis by slit-lamp examination of infected eyes. RESULTS: Antibody was not produced after PIV immunization but was induced by rPIV. Rabbits immunized first with soluble and then precipitated rPIV produced high titers (log(10)) to rPIV (4.28 +/- 0.09) and significantly higher titers to PIV (3.90 +/- 0.06) compared to the other immunized groups. Antibody to rPIV reacted with PIV, but neither neutralized enzyme activity in vitro nor protected infected rabbits in vivo. CONCLUSIONS: The present study demonstrates that PIV is a virulence factor which can escape a protective immune response.
Authors: Aihua Tang; Mary E Marquart; Jonathan D Fratkin; Clare C McCormick; Armando R Caballero; Hattie P Gatlin; Richard J O'Callaghan Journal: Invest Ophthalmol Vis Sci Date: 2009-02-28 Impact factor: 4.799
Authors: Jessica L Bradshaw; Armando R Caballero; Michael A Bierdeman; Kristen V Adams; Haley R Pipkins; Aihua Tang; Richard J O'Callaghan; Larry S McDaniel Journal: mSphere Date: 2018-05-02 Impact factor: 4.389
Authors: Luke L Proctor; Whitney L Ward; Conner S Roggy; Alexandra G Koontz; Katie M Clark; Alyssa P Quinn; Meredith Schroeder; Amanda E Brooks; James M Small; Francina D Towne; Benjamin D Brooks Journal: Antibiotics (Basel) Date: 2021-12-14