SETTING: The Ural region in Russia is one of the areas most affected by a high incidence of tuberculosis (TB). Molecular epidemiological studies able to trace Mycobacterium tuberculosis transmission are of particular significance. OBJECTIVE: To characterize the population of M. tuberculosis strains circulating in the Ural region, to detect the predominant genotypes and to evaluate their phylogenetic relationship and epidemiological significance. DESIGN: Ninety-two M. tuberculosis clinical samples originating from the Ural region were genotyped using the MIRU-VNTR method. RESULTS: Two major phylogenetically distinct groups of isolates were identified: the W-Beijing family (54.3%) and a previously unreported cluster, named the Ural group (15.2%). Forty-seven different MIRU profiles were identified, including 38 unique (41.3%) and 54 isolates grouped into nine clusters (from 2 to 28 isolates in each cluster). Genetic diversity within the clusters was shown by additional sub-typing of M. tuberculosis isolates in nine additional QUB-VNTR loci. CONCLUSION: W-Beijing family isolates are associated with multiresistance to anti-tuberculosis drugs. It is possible that the strains of this family play a significant role in the spread of multidrug-resistant TB over the Ural region.
SETTING: The Ural region in Russia is one of the areas most affected by a high incidence of tuberculosis (TB). Molecular epidemiological studies able to trace Mycobacterium tuberculosis transmission are of particular significance. OBJECTIVE: To characterize the population of M. tuberculosis strains circulating in the Ural region, to detect the predominant genotypes and to evaluate their phylogenetic relationship and epidemiological significance. DESIGN: Ninety-two M. tuberculosisclinical samples originating from the Ural region were genotyped using the MIRU-VNTR method. RESULTS: Two major phylogenetically distinct groups of isolates were identified: the W-Beijing family (54.3%) and a previously unreported cluster, named the Ural group (15.2%). Forty-seven different MIRU profiles were identified, including 38 unique (41.3%) and 54 isolates grouped into nine clusters (from 2 to 28 isolates in each cluster). Genetic diversity within the clusters was shown by additional sub-typing of M. tuberculosis isolates in nine additional QUB-VNTR loci. CONCLUSION: W-Beijing family isolates are associated with multiresistance to anti-tuberculosis drugs. It is possible that the strains of this family play a significant role in the spread of multidrug-resistant TB over the Ural region.
Authors: E Chernyaeva; P Dobrynin; N Pestova; N Matveeva; V Zhemkov; A Kozlov Journal: Eur J Clin Microbiol Infect Dis Date: 2011-12-08 Impact factor: 3.267
Authors: M V Afanas'ev; L N Ikryannikova; E N Il'ina; A V Kuz'min; E E Larionova; T G Smirnova; L N Chernousova; V M Govorun Journal: Eur J Clin Microbiol Infect Dis Date: 2010-10-13 Impact factor: 3.267
Authors: Maya A Dymova; Oleksander O Liashenko; Petro I Poteiko; Valeriy S Krutko; Eugeny A Khrapov; Maxim L Filipenko Journal: BMC Infect Dis Date: 2011-03-28 Impact factor: 3.090
Authors: Igor Mokrousov; Anna Vyazovaya; Tatiana Otten; Viacheslav Zhuravlev; Elena Pavlova; Larisa Tarashkevich; Valery Krishevich; Boris Vishnevsky; Olga Narvskaya Journal: PLoS One Date: 2012-07-23 Impact factor: 3.240