Peng Shen1, Lei Xia, Hai-jiang Zhang. 1. The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310003, China. shenp@zju.edu.cn
Abstract
OBJECTIVE: To study the main active constituents of Siwu mixture for reversing the multidrug resistance (MDR) of human erythrocyte leukemic cell line K562/ADM. METHOD: Macroporous resin was used to separate Siwu mixture and an in vitro model was used to evaluate the reversal index of each fraction for discovery of active fraction. HPLC-MS was used for investigating the main active constituents of Siwu mixture. RESULT: The reversal index of the gross polysaccharide of Rehmannia glutinosa was significantly higher than that of blank control (P < 0.01). There was little difference between the reversal effect of the gross polysaccharide of Rehmannia glutinosa and that of the active fraction A (P > 0.05). CONCLUSION: The gross polysaccharide of Rehmannia glutinosa is one of the main active constituents of Siwu mixture.
OBJECTIVE: To study the main active constituents of Siwu mixture for reversing the multidrug resistance (MDR) of human erythrocyte leukemic cell line K562/ADM. METHOD: Macroporous resin was used to separate Siwu mixture and an in vitro model was used to evaluate the reversal index of each fraction for discovery of active fraction. HPLC-MS was used for investigating the main active constituents of Siwu mixture. RESULT: The reversal index of the gross polysaccharide of Rehmannia glutinosa was significantly higher than that of blank control (P < 0.01). There was little difference between the reversal effect of the gross polysaccharide of Rehmannia glutinosa and that of the active fraction A (P > 0.05). CONCLUSION: The gross polysaccharide of Rehmannia glutinosa is one of the main active constituents of Siwu mixture.
Authors: Jian-Shu Lou; Lu Yan; Cathy W C Bi; Gallant K L Chan; Qi-Yun Wu; Yun-Le Liu; Yun Huang; Ping Yao; Crystal Y Q Du; Tina T X Dong; Karl W K Tsim Journal: Sci Rep Date: 2016-08-25 Impact factor: 4.379