BACKGROUND: We previously reported on the high propensity of pathogenic oral flora in the oropharynx of nasogastric tube-fed patients, and subsequently showed biofilm formation on the NGTs of these patients. There is a close relationship of biofilm and oropharyngeal colonization with pathogenic bacteria, aspiration pneumonia and antibiotic resistance. OBJECTIVES: To investigate the time relation between the insertion of a new NGT and formation of the biofilm. METHODS: We examined sequential samples on NGTs that were forcibly pulled out by the patients themselves during any of the 7 days after insertion. Scanning electron micrography and confocal laser scanning microscopy were used for biofilm detection. RESULTS: Biofilm was identified on 60% of the five samples of day 1 and on all the samples of the following days, by both microscopic methods. CONCLUSIONS: Biofilms form within a single day on most NGTs inserted for the feeding of elderly patients with dysphagia. Further research should be devoted to prevention of biofilm formation on NGTs.
BACKGROUND: We previously reported on the high propensity of pathogenic oral flora in the oropharynx of nasogastric tube-fed patients, and subsequently showed biofilm formation on the NGTs of these patients. There is a close relationship of biofilm and oropharyngeal colonization with pathogenic bacteria, aspiration pneumonia and antibiotic resistance. OBJECTIVES: To investigate the time relation between the insertion of a new NGT and formation of the biofilm. METHODS: We examined sequential samples on NGTs that were forcibly pulled out by the patients themselves during any of the 7 days after insertion. Scanning electron micrography and confocal laser scanning microscopy were used for biofilm detection. RESULTS: Biofilm was identified on 60% of the five samples of day 1 and on all the samples of the following days, by both microscopic methods. CONCLUSIONS: Biofilms form within a single day on most NGTs inserted for the feeding of elderly patients with dysphagia. Further research should be devoted to prevention of biofilm formation on NGTs.
Authors: Erik C von Rosenvinge; Graeme A O'May; Sandra Macfarlane; George T Macfarlane; Mark E Shirtliff Journal: Pathog Dis Date: 2013-01-29 Impact factor: 3.166
Authors: Leslie A Parker; Marina Magalhães; Katelyn Desorcy-Scherer; Monica Torrez Lamberti; Graciela L Lorca; Josef Neu Journal: Front Nutr Date: 2022-02-24