| Literature DB >> 16008110 |
Christoph Hundhausen1, Christine Bösch-Saadatmandi, Kay Augustin, Ralf Blank, Siegfried Wolffram, Gerald Rimbach.
Abstract
It has been shown that oxidative damage contributes to the wide range of toxic effects of the mycotoxin ochratoxin A (OTA). Therefore, we examined the effects of alpha-tocopherol (alpha-TOC) and different polyphenols--catechin (CAT), daidzein (DAI), epicatechin (EC), epigallocatechin gallate (EGCG), genistein (GEN), and quercetin (QUE)--on OTA-induced cytotoxicity in HepG2 liver cells. Incubation of HepG2 cells with increasing concentrations of OTA resulted in a dose- and time-dependent cytotoxicity as measured by the neutral red assay. Half lethal concentrations (LC50) of OTA were 35 and 10 microM after 48 and 72 h incubation, respectively. Incubation of HepG2 cells with alpha-TOC as well as with different polyphenols (exhibiting different antioxidant potency as determined by the FRAP, TEAC and DPPH assays) did not counteract OTA-induced cytotoxicity. These findings indicate that OTA may exert its toxic effects by affecting other hepatic mechanisms than those directly modulated by alpha-TOC and polyphenols.Entities:
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Year: 2005 PMID： 16008110 DOI： 10.1016/j.jplph.2005.04.021
Source DB: PubMed Journal: J Plant Physiol ISSN： 0176-1617 Impact factor: 3.549