Literature DB >> 16006601

Technical aspects of immunohistochemistry.

J A Ramos-Vara1.   

Abstract

Immunohistochemistry is an integral technique in many veterinary laboratories for diagnostic and research purposes. In the last decade, the ability to detect antigens (Ags) in tissue sections has improved dramatically, mainly by countering the deleterious effects of formaldehyde with antigen retrieval (AR) and increasing sensitivity of the detection systems. In this review, I address these topics and provide an overview of technical aspects of immunohistochemistry, including those related to antibodies (Abs) and Ags, fixation, AR, detection methods, background, and troubleshooting. Microarray technology and the use of rabbit monoclonal Abs in immunohistochemistry are also discussed.

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Year:  2005        PMID： 16006601     DOI： 10.1354/vp.42-4-405

Source DB:  PubMed          Journal:  Vet Pathol        ISSN： 0300-9858            Impact factor:   2.221


  158 in total

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