| Literature DB >> 16006095 |
Paola Cremonesi1, Massimo Luzzana, Milena Brasca, Stefano Morandi, Roberta Lodi, Chiara Vimercati, Dario Agnellini, Giancarlo Caramenti, Paolo Moroni, Bianca Castiglioni.
Abstract
A multiplex PCR for the simultaneous detection of Staphylococcus aureus 23S rRNA, the coagulase and thermonuclease genes as well as the enterotoxin genes sea, sec, sed, seg, seh, sei, sej, sel was developed. The method was used to determine the presence of enterotoxigenic types for 93 S. aureus strains isolated from milk and dairy products. The data obtained by mPCR resulted comparable to those obtained by immunoassay methods. In addition, the mPCR assays also amplified some se genes, whose toxins are undetectable by immunoassay. Multiplex amplification can be obtained starting from 1 pg of DNA, showing the excellent specificity and high sensitivity of the assay.Entities:
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Year: 2005 PMID： 16006095 DOI： 10.1016/j.mcp.2005.03.002
Source DB: PubMed Journal: Mol Cell Probes ISSN： 0890-8508 Impact factor: 2.365