Literature DB >> 16002184

A modified pulsed-field gel electrophoresis (PFGE) protocol for subtyping previously non-PFGE typeable isolates of Clostridium difficile polymerase chain reaction ribotype 001.

M Gal1, G Northey, J S Brazier.   

Abstract

A modified pulsed-field gel electrophoresis (PFGE) protocol was developed and applied to 50 isolates of the UK epidemic strain of Clostridium difficile, polymerase chain reaction (PCR) ribotype 001, to develop a PFGE-based subtyping scheme. This protocol overcame the inherent DNA degradation problems associated with typing this strain of C. difficile by this method, and whole genomic digestion with SmaI restriction enzyme yielded seven distinct and reproducible PFGE banding patterns. Modified PFGE is an appropriate method for subtyping C. difficile PCR ribotype 001 that could be used to improve epidemiological investigations.

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Year:  2005        PMID： 16002184     DOI： 10.1016/j.jhin.2005.01.017

Source DB:  PubMed          Journal:  J Hosp Infect        ISSN： 0195-6701            Impact factor:   3.926


  9 in total

1.  Molecular analysis of Clostridium difficile PCR ribotype 027 isolates from Eastern and Western Canada.

Authors:  Duncan R MacCannell; Thomas J Louie; Dan B Gregson; Michel Laverdiere; Annie-Claude Labbe; Felicia Laing; Scott Henwick
Journal:  J Clin Microbiol       Date:  2006-06       Impact factor: 5.948

2.  Comparative phylogenomics of Clostridium difficile reveals clade specificity and microevolution of hypervirulent strains.

Authors:  R A Stabler; D N Gerding; J G Songer; D Drudy; J S Brazier; H T Trinh; A A Witney; J Hinds; B W Wren
Journal:  J Bacteriol       Date:  2006-10       Impact factor: 3.490

3.  DNA microarray-based PCR ribotyping of Clostridium difficile.

Authors:  Alexander Schneeberg; Ralf Ehricht; Peter Slickers; Vico Baier; Heinrich Neubauer; Stefan Zimmermann; Denise Rabold; Antina Lübke-Becker; Christian Seyboldt
Journal:  J Clin Microbiol       Date:  2014-11-19       Impact factor: 5.948

4.  Molecular analysis of Clostridium difficile at a university teaching hospital in Japan: a shift in the predominant type over a five-year period.

Authors:  E Sawabe; H Kato; K Osawa; T Chida; N Tojo; Y Arakawa; N Okamura
Journal:  Eur J Clin Microbiol Infect Dis       Date:  2007-10       Impact factor: 3.267

5.  Genetic organisation, mobility and predicted functions of genes on integrated, mobile genetic elements in sequenced strains of Clostridium difficile.

Authors:  Michael S M Brouwer; Philip J Warburton; Adam P Roberts; Peter Mullany; Elaine Allan
Journal:  PLoS One       Date:  2011-08-18       Impact factor: 3.240

6.  Comparison of PCR ribotyping and multilocus variable-number tandem-repeat analysis (MLVA) for improved detection of Clostridium difficile.

Authors:  Hsiao L Wei; Chun Wei Kao; Sung H Wei; Jason T C Tzen; Chien S Chiou
Journal:  BMC Microbiol       Date:  2011-09-30       Impact factor: 3.605

7.  Molecular epidemiology and antimicrobial susceptibility of Clostridium difficile isolates from two Korean hospitals.

Authors:  Asiimwe Nicholas; Yu Kyung Kim; Won-Kil Lee; Gati Noble Selasi; Seok Hyeon Na; Hyo Il Kwon; Yoo Jeong Kim; Hae Sook Lee; Kyung Eun Song; Jeong Hwan Shin; Je Chul Lee
Journal:  PLoS One       Date:  2017-03-29       Impact factor: 3.240

8.  Characterization of Clostridium difficile isolates using capillary gel electrophoresis-based PCR ribotyping.

Authors:  A Indra; S Huhulescu; M Schneeweis; P Hasenberger; S Kernbichler; A Fiedler; G Wewalka; F Allerberger; E J Kuijper
Journal:  J Med Microbiol       Date:  2008-11       Impact factor: 2.472

9.  Typing Clostridium difficile strains based on tandem repeat sequences.

Authors:  N Henning Zaiss; Maja Rupnik; Ed J Kuijper; Celine Harmanus; Dolf Michielsen; Koen Janssens; Ulrich Nübel
Journal:  BMC Microbiol       Date:  2009-01-08       Impact factor: 3.605
  9 in total

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