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Literature DB >> 16000486

Multiplex PCR for identification of seven Streptococcus pneumoniae serotypes targeted by a 7-valent conjugate vaccine.

Abstract

Here we describe a Streptococcus pneumoniae one-step multiplex PCR assay which identifies by amplicon size the seven capsular polysaccharide serotypes targeted by the 7-valent conjugate vaccine. The multiplex PCR assay was used to blindly assay clinical isolates recovered during 1999 in the Republic of Ireland from cases of invasive disease whose serotypes were previously determined by classical methods.

Entities: Chemical Disease Species

Mesh：

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Year: 2005 PMID： 16000486 PMCID： PMC1169178 DOI： 10.1128/JCM.43.7.3487-3490.2005

Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948

18 in total

1. Pneumococcal disease and the role of conjugate vaccines.

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Journal: Microb Drug Resist Date: 1999 Impact factor: 3.431

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Authors: S F Altschul; W Gish; W Miller; E W Myers; D J Lipman
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Journal: Nucleic Acids Res Date: 1997-12-15 Impact factor: 16.971

4. Effect of genetic switching of capsular type on virulence of Streptococcus pneumoniae.

Authors: T Kelly; J P Dillard; J Yother
Journal: Infect Immun Date: 1994-05 Impact factor: 3.441

5. The burden of severe pneumococcal infection in Ireland: potential effectiveness and indirect benefits of the 7-valent pneumococcal conjugate vaccine.

Authors: S McCormack; E D G McIntosh
Journal: Ir Med J Date: 2004-10

6. Global distribution of Streptococcus pneumoniae serotypes isolated from paediatric patients during 1999-2000 and the in vitro efficacy of telithromycin and comparators.

Authors: D J Farrell; S G Jenkins; R R Reinert
Journal: J Med Microbiol Date: 2004-11 Impact factor: 2.472

Review 7. [Clinical effectiveness of seven-valent pneumococcal conjugate vaccine (Prevenar) against invasive pneumococcal diseases: prospects for children in France].

Authors: S Black; H Shinefield; R Cohen; D Floret; J Gaudelus; C Olivier; P Reinert
Journal: Arch Pediatr Date: 2004-07 Impact factor: 1.180

8. Pneumococcal polysaccharide vaccine efficacy. An evaluation of current recommendations.

Authors: J C Butler; R F Breiman; J F Campbell; H B Lipman; C V Broome; R R Facklam
Journal: JAMA Date: 1993-10-20 Impact factor: 56.272

9. The 23-valent pneumococcal polysaccharide vaccine. Part I. Efficacy of PPV in the elderly: a comparison of meta-analyses.

Authors: Alessia Melegaro; W John Edmunds
Journal: Eur J Epidemiol Date: 2004 Impact factor: 8.082

10. Penicillin susceptibility and epidemiological typing of invasive pneumococcal isolates in the Republic of Ireland.

Authors: D Bennett; B Lennon; H Humphreys; M Cafferkey
Journal: J Clin Microbiol Date: 2003-08 Impact factor: 5.948

15 in total

1. Multiplex PCR to determine Streptococcus pneumoniae serotypes causing otitis media in the Republic of Ireland with further characterisation of antimicrobial susceptibilities and genotypes.

Authors: I Vickers; D O'Flanagan; M Cafferkey; H Humphreys
Journal: Eur J Clin Microbiol Infect Dis Date: 2010-11-13 Impact factor: 3.267

2. Serotype distribution and antimicrobial susceptibilities of nasopharyngeal isolates of Streptococcus pneumoniae from children hospitalized for acute respiratory illnesses in Hong Kong.

Authors: Margaret Ip; E Anthony S Nelson; Edmund S C Cheuk; Rita Y T Sung; Albert Li; Helen Ma; Paul K S Chan
Journal: J Clin Microbiol Date: 2007-04-04 Impact factor: 5.948

10. Molecular detection methods and serotyping performed directly on clinical samples improve diagnostic sensitivity and reveal increased incidence of invasive disease by Streptococcus pneumoniae in Italian children.

Authors: Chiara Azzari; Maria Moriondo; Giuseppe Indolfi; Cristina Massai; Laura Becciolini; Maurizio de Martino; Massimo Resti
Journal: J Med Microbiol Date: 2008-10 Impact factor: 2.472

Multiplex PCR for identification of seven Streptococcus pneumoniae serotypes targeted by a 7-valent conjugate vaccine.

1. Pneumococcal disease and the role of conjugate vaccines.

2. Basic local alignment search tool.

3. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.

4. Effect of genetic switching of capsular type on virulence of Streptococcus pneumoniae.

5. The burden of severe pneumococcal infection in Ireland: potential effectiveness and indirect benefits of the 7-valent pneumococcal conjugate vaccine.

6. Global distribution of Streptococcus pneumoniae serotypes isolated from paediatric patients during 1999-2000 and the in vitro efficacy of telithromycin and comparators.

Review 7. [Clinical effectiveness of seven-valent pneumococcal conjugate vaccine (Prevenar) against invasive pneumococcal diseases: prospects for children in France].

8. Pneumococcal polysaccharide vaccine efficacy. An evaluation of current recommendations.

9. The 23-valent pneumococcal polysaccharide vaccine. Part I. Efficacy of PPV in the elderly: a comparison of meta-analyses.

10. Penicillin susceptibility and epidemiological typing of invasive pneumococcal isolates in the Republic of Ireland.

1. Multiplex PCR to determine Streptococcus pneumoniae serotypes causing otitis media in the Republic of Ireland with further characterisation of antimicrobial susceptibilities and genotypes.

2. Serotype distribution and antimicrobial susceptibilities of nasopharyngeal isolates of Streptococcus pneumoniae from children hospitalized for acute respiratory illnesses in Hong Kong.

3. Sequetyping: serotyping Streptococcus pneumoniae by a single PCR sequencing strategy.

4. Simple, accurate, serotype-specific PCR assay to differentiate Streptococcus pneumoniae serotypes 6A, 6B, and 6C.

5. Usefulness of Pneumotest-latex for direct serotyping of Streptococcus pneumoniae isolates in clinical samples.

6. Use of miniaturized protein arrays for Escherichia coli O serotyping.

7. Single-Step Multiplex PCR Assay for Determining 92 Pneumococcal Serotypes.

8. Development of an automated and multiplexed serotyping assay for Streptococcus pneumoniae.

9. Validation of a multiplex pneumococcal serotyping assay with clinical samples.

10. Molecular detection methods and serotyping performed directly on clinical samples improve diagnostic sensitivity and reveal increased incidence of invasive disease by Streptococcus pneumoniae in Italian children.