| Literature DB >> 16000478 |
K F Laserson1, N T N Yen, C G Thornton, V T C Mai, W Jones, D Q An, N H Phuoc, N A Trinh, D T C Nhung, T X Lien, N T N Lan, C Wells, N Binkin, M Cetron, S A Maloney.
Abstract
The goal of this study was to evaluate the effect of the specimen-processing method that uses the detergent C18-carboxypropylbetaine (CB-18) on the sensitivity of acid-fast bacillus (AFB) staining. Vietnamese immigrants with abnormal chest radiographs provided up to three sputum specimens, which were examined for acid-fast bacilli by use of direct auramine and Ziehl-Neelsen staining. The remaining sputum was split; half was cultured, and the other half was incubated with CB-18 for 24 h, centrifuged, and examined for AFB by both staining methods. CB-18 processing improved the sensitivity of AFB staining by 20 to 30% (only differences in auramine sensitivity were statistically significant) but reduced specificity by approximately 20% (P < 0.05). These findings have direct utility for overseas migrant tuberculosis screening programs, for which maximizing test sensitivity is a major objective.Entities:
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Year: 2005 PMID： 16000478 PMCID： PMC1169166 DOI： 10.1128/JCM.43.7.3460-3462.2005
Source DB: PubMed Journal: J Clin Microbiol ISSN： 0095-1137 Impact factor: 5.948