Literature DB >> 16000473

Rapid detection of human metapneumovirus strains in nasopharyngeal aspirates and shell vial cultures by monoclonal antibodies.

Elena Percivalle1, Antonella Sarasini, Livia Visai, M Grazia Revello, Giuseppe Gerna.   

Abstract

Monoclonal antibodies to human metapneumovirus (hMPV) were developed for direct fluorescent antibody (DFA) staining of nasopharyngeal aspirates from 40 infants with respiratory infections, as well as for hMPV identification in shell vial cultures. With reference to reverse transcription-PCR, DFA staining showed sensitivity, specificity, and positive and negative predictive values of 73.9%, 94.1%, 94.4%, and 72.7%, respectively. Monoclonal antibodies are useful for direct hMPV detection.

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Year:  2005        PMID： 16000473      PMCID： PMC1169090          DOI： 10.1128/JCM.43.7.3443-3446.2005

Source DB:  PubMed          Journal:  J Clin Microbiol        ISSN： 0095-1137            Impact factor:   5.948


  13 in total

1.  Virological features and clinical manifestations associated with human metapneumovirus: a new paramyxovirus responsible for acute respiratory-tract infections in all age groups.

Authors:  Guy Boivin; Yacine Abed; Gilles Pelletier; Louisette Ruel; Danielle Moisan; Stéphanie Côté; Teresa C T Peret; Dean D Erdman; Larry J Anderson
Journal:  J Infect Dis       Date:  2002-10-08       Impact factor: 5.226

2.  Subtype B avian metapneumovirus resembles subtype A more closely than subtype C or human metapneumovirus with respect to the phosphoprotein, and second matrix and small hydrophobic proteins.

Authors:  Janet Ashley Jacobs; M Kariuki Njenga; Rene Alvarez; Karen Mawditt; Paul Britton; Dave Cavanagh; Bruce S Seal
Journal:  Virus Res       Date:  2003-04       Impact factor: 3.303

3.  Use of the P gene to genotype human metapneumovirus identifies 4 viral subtypes.

Authors:  Ian M Mackay; Seweryn Bialasiewicz; Zubair Waliuzzaman; Glenys R Chidlow; David C Fegredo; Somprasong Laingam; Penny Adamson; Gerald B Harnett; William Rawlinson; Michael D Nissen; Theo P Sloots
Journal:  J Infect Dis       Date:  2004-11-03       Impact factor: 5.226

Review 4.  Metapneumoviruses in birds and humans.

Authors:  M Kariuki Njenga; Humphrey M Lwamba; Bruce S Seal
Journal:  Virus Res       Date:  2003-02       Impact factor: 3.303

5.  Human metapneumovirus and lower respiratory tract disease in otherwise healthy infants and children.

Authors:  John V Williams; Paul A Harris; Sharon J Tollefson; Lisa L Halburnt-Rush; Joyce M Pingsterhaus; Kathryn M Edwards; Peter F Wright; James E Crowe
Journal:  N Engl J Med       Date:  2004-01-29       Impact factor: 91.245

6.  A newly discovered human pneumovirus isolated from young children with respiratory tract disease.

Authors:  B G van den Hoogen; J C de Jong; J Groen; T Kuiken; R de Groot; R A Fouchier; A D Osterhaus
Journal:  Nat Med       Date:  2001-06       Impact factor: 53.440

7.  Antigenic and genetic variability of human metapneumoviruses.

Authors:  Bernadette G van den Hoogen; Sander Herfst; Leo Sprong; Patricia A Cane; Eduardo Forleo-Neto; Rik L de Swart; Albert D M E Osterhaus; Ron A M Fouchier
Journal:  Emerg Infect Dis       Date:  2004-04       Impact factor: 6.883

8.  Human metapneumovirus detection in patients with severe acute respiratory syndrome.

Authors:  Paul K S Chan; John S Tam; Ching-Wan Lam; Edward Chan; Alan Wu; Chi-Kong Li; Thomas A Buckley; King-Cheung Ng; Gavin M Joynt; Frankie W T Cheng; Ka-Fai To; Nelson Lee; David S C Hui; Jo L K Cheung; Ida Chu; Esther Liu; Sydney S C Chung; Joseph J Y Sung
Journal:  Emerg Infect Dis       Date:  2003-09       Impact factor: 6.883

9.  Global genetic diversity of human metapneumovirus fusion gene.

Authors:  Guy Boivin; Ian Mackay; Theo P Sloots; Shabir Madhi; François Freymuth; Dana Wolf; Yonat Shemer-Avni; Herbert Ludewick; Gregory C Gray; Eric LeBlanc
Journal:  Emerg Infect Dis       Date:  2004-06       Impact factor: 6.883

10.  Sequence analysis of the N, P, M and F genes of Canadian human metapneumovirus strains.

Authors:  Nathalie Bastien; Susan Normand; Tracy Taylor; Diane Ward; Teresa C T Peret; Guy Boivin; Larry J Anderson; Yan Li
Journal:  Virus Res       Date:  2003-05       Impact factor: 3.303
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  22 in total

1.  Human metapneumovirus infections are associated with severe morbidity in hospitalized children of all ages.

Authors:  A Hahn; W Wang; P Jaggi; I Dvorchik; O Ramilo; K Koranyi; A Mejias
Journal:  Epidemiol Infect       Date:  2013-01-07       Impact factor: 2.451

2.  Rates of hospitalizations for respiratory syncytial virus, human metapneumovirus, and influenza virus in older adults.

Authors:  Kyle Widmer; Yuwei Zhu; John V Williams; Marie R Griffin; Kathryn M Edwards; H Keipp Talbot
Journal:  J Infect Dis       Date:  2012-04-23       Impact factor: 5.226

Review 3.  Epidemiology of human metapneumovirus.

Authors:  Jeffrey S Kahn
Journal:  Clin Microbiol Rev       Date:  2006-07       Impact factor: 26.132

Review 4.  Detection of respiratory viruses by molecular methods.

Authors:  James B Mahony
Journal:  Clin Microbiol Rev       Date:  2008-10       Impact factor: 26.132

5.  Evaluation of a new rapid antigen test using immunochromatography for detection of human metapneumovirus in comparison with real-time PCR assay.

Authors:  Yoko Matsuzaki; Emi Takashita; Michiko Okamoto; Katsumi Mizuta; Tsutomu Itagaki; Fumio Katsushima; Yuriko Katsushima; Yukio Nagai; Hidekazu Nishimura
Journal:  J Clin Microbiol       Date:  2009-07-01       Impact factor: 5.948

6.  Prospective evaluation of rapid antigen tests for diagnosis of respiratory syncytial virus and human metapneumovirus infections.

Authors:  Jaber Aslanzadeh; Xiaotian Zheng; Haijing Li; Janice Tetreault; Irene Ratkiewicz; Shufang Meng; Pamela Hamilton; Yi-Wei Tang
Journal:  J Clin Microbiol       Date:  2008-03-12       Impact factor: 5.948

7.  Outbreak of human metapneumovirus detected by use of the Vero E6 cell line in isolates collected in Yamagata, Japan, in 2004 and 2005.

Authors:  C Abiko; K Mizuta; T Itagaki; N Katsushima; S Ito; Y Matsuzaki; M Okamoto; H Nishimura; Y Aoki; T Murata; H Hoshina; S Hongo; K Ootani
Journal:  J Clin Microbiol       Date:  2007-04-25       Impact factor: 5.948

8.  Prospective study of human metapneumovirus detection in clinical samples by use of light diagnostics direct immunofluorescence reagent and real-time PCR.

Authors:  Marie L Landry; Sandra Cohen; David Ferguson
Journal:  J Clin Microbiol       Date:  2008-01-09       Impact factor: 5.948

9.  Human metapneumovirus (hMPV) in acute respiratory infection: a clinic-based study in Egypt.

Authors:  Sohier Yahia; Amina Y Kandeel; Enas Hammad; Abdel-Hady El-Gilany
Journal:  Indian J Pediatr       Date:  2012-10       Impact factor: 1.967

Review 10.  [Application of molecular methods in the diagnosis and epidemiological study of viral respiratory infections].

Authors:  Francisco Pozo; Inmaculada Casas; Guillermo Ruiz; Ana Falcón; Pilar Pérez-Breña
Journal:  Enferm Infecc Microbiol Clin       Date:  2008-07       Impact factor: 1.731
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