AIM: To investigate the radiation response of various human tumor cells and normal liver cells. METHODS: Cell lines of human hepatoma cells (SMMC-7721), liver cells (L02), melanoma cells (A375) and cervical tumor (HeLa) were irradiated with (60)Co gamma-rays. Cell survive was documented by a colony assay. Chromatid breaks were measured by counting the number of chromatid breaks and isochromatid breaks immediately after prematurely chromosome condensed by Calyculin-A. RESULTS: Linear quadratic survival curve was observed in all of four cell lines, and dose-dependent increase in radiation-induced chromatid and isochromatid breaks were observed in GB2B phase. Among these four cell lines, A375 was most sensitive to radiation, while, L02 had the lowest radiosensitivity. For normal liver cells, chromatid breaks were easy to be repaired, isochromatid breaks were difficult to be repaired. CONCLUSION: The results suggest that the gamma-rays induced chromatid breaks can be possibly used as a good predictor of radiosensitivity, also, unrejoined isochromatid breaks probably tightly related with cell cancerization.
AIM: To investigate the radiation response of various humantumor cells and normal liver cells. METHODS: Cell lines of humanhepatoma cells (SMMC-7721), liver cells (L02), melanoma cells (A375) and cervical tumor (HeLa) were irradiated with (60)Co gamma-rays. Cell survive was documented by a colony assay. Chromatid breaks were measured by counting the number of chromatid breaks and isochromatid breaks immediately after prematurely chromosome condensed by Calyculin-A. RESULTS: Linear quadratic survival curve was observed in all of four cell lines, and dose-dependent increase in radiation-induced chromatid and isochromatid breaks were observed in GB2B phase. Among these four cell lines, A375 was most sensitive to radiation, while, L02 had the lowest radiosensitivity. For normal liver cells, chromatid breaks were easy to be repaired, isochromatid breaks were difficult to be repaired. CONCLUSION: The results suggest that the gamma-rays induced chromatid breaks can be possibly used as a good predictor of radiosensitivity, also, unrejoined isochromatid breaks probably tightly related with cell cancerization.
Authors: M Durante; Y Furusawa; K George; G Gialanella; O Greco; G Grossi; N Matsufuji; M Pugliese; T C Yang Journal: Radiat Res Date: 1998-05 Impact factor: 2.841
Authors: J M Coco Martin; E Mooren; C Ottenheim; W Burrill; M I Nunez; D Sprong; H Bartelink; A C Begg Journal: Int J Radiat Biol Date: 1999-09 Impact factor: 2.694
Authors: Bruno F Fernandes; Alexandre N Odashiro; Vinicius S Saraiva; Patrick Logan; Emilia Antecka; Miguel N Burnier Journal: J Carcinog Date: 2007-04-20
Authors: Xing Guo Wang; Ekaterina Revskaya; Ruth A Bryan; Howard D Strickler; Robert D Burk; Arturo Casadevall; Ekaterina Dadachova Journal: PLoS One Date: 2007-10-31 Impact factor: 3.240